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조회 3451  스크랩 인쇄하기 주소복사 트위터 공유 페이스북 공유 
질문 Lentiviral plasmid sequence 요청드립니다.
알실험생10(대학원생)  |  09.19 17:10

안녕하세요. Lentiviral plasmid sequence요청드립니다.

 

Systembio에서 판매하는 CD513-B1 vector sequence 부탁드립니다.

 

정말 제발 부탁드립니다ㅠㅠ 

 

nyj1350@gmail.com로 보내주시면 감사하겠습니다.

 

 제발 부탁드립니다ㅠㅠ

#Plasmid
 
#pCDH
답변하기
답변 본 정보는 네티즌에 의해 작성된 정보로, 내용 중 중요하다고 생각되는 부분은 추가적인 사실 확인을 반드시 하시길 바랍니다.

염기서열이 여러개가 있는데 7,377bp / 7,384bp 중 어느 bp인가요?

좀더 확인해 보겠습니다.

대왕개구리SPEED  |  09.19 18:35  
혹시 둘 다 있으시다면 알려주실 수 있으실까요?? 제발 부탁드립니다ㅠㅠ
알실험생10  |  09.19 19:22  
CD513-B1의 greenpuro가 있는 sequence가 맞으실까요..? 제가 알기로 더 큰 size로 알고 있는데 확인해주실 수 있으실까요? 부탁드립니다ㅠㅠ
알실험생10  |  09.19 20:13  
채택답변: 질문자가 채택한 답변입니다.

CD513B-1 plasmid sequence입니다.

8201bp

        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgaatt taaatcggat ccgcggccgc gaaggatctg cgatcgctcc
     2341 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     2401 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     2461 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     2521 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     2581 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     2641 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     2701 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     2761 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     2821 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatggagag
     2881 cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg
     2941 cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa
     3001 caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat
     3061 gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca
     3121 cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt
     3181 gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt
     3241 ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa
     3301 cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg
     3361 caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt
     3421 caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg
     3481 cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa
     3541 gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac
     3601 cgccggaccc ggctccaccg gatctcgctg agggcagagg aagtcttcta acatgcggtg
     3661 acgtggagga gaatcccggc ccttccggga tgaccgagta caagcccacg gtgcgcctcg
     3721 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3781 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     3841 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     3901 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     3961 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     4021 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     4081 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     4141 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     4201 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4261 ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt
     4321 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4381 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4441 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4501 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4561 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4621 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4681 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4741 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4801 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4861 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     4921 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4981 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     5041 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5101 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5161 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     5221 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     5281 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5341 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     5401 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5461 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5521 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     5581 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5641 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5701 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5761 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5821 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5881 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5941 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6001 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6061 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6121 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6181 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6241 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6301 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6361 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6421 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6481 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6541 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6601 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6661 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6721 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6781 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6841 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6901 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6961 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7021 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7081 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7141 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7201 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7261 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7321 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7381 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7441 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7501 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7561 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7621 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7681 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7741 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     7801 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7861 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7921 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7981 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     8041 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     8101 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     8161 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
 

대왕개구리SPEED  |  09.19 20:33  
정말 감사합니다ㅠㅠ 진짜 너무 감사합니다ㅠ
알실험생10  |  09.19 20:59  

선생님 어제 답변 주신 sequence에서 GFP가 CopGFP인 것 같아서 혹시 CMV promotor에 일반 GFP와 Puromycin resistance gene이 있는 CD513-B1 vector sequence가 있으실까요?

확인 부탁드립니다ㅠㅠ

 

cd513-b1에 대한 이미지 검색결과

알실험생10  |  09.20 16:34  

제품 번호가 CD513-B1이 아니라 CD513B-1입니다.

SBI 제품 중에 copGFP가 있는 제품은 Puro gene이 없는 것만 있습니다.

혹시 copGFP + Puro가 들어있는 vector가 있나요?

답변에 올린 서열은 Puro gene이 들어있고 CD513B-1이 맞습니다.

아래 첨부한 것은 서열에 나와있는 제품 번호입니다.

upload_image

대왕개구리SPEED  |  09.20 18:27  
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