실험 Q&A > Etc.
Carbohydrate FITC labelling 질문
레벨1 유기견 (대학원생)
제가 만든 carbohydrate에 FITC를 붙이고 싶은데
Protein에 붙이는 protocol을 그대로 따라도 괜찮을까요??
여기서 찾아본 protocol은
1. Prepare antibody (1.5 mg/ml)
2. Dialyze antibody against 50mM Carbonate Buffer, pH11.6
3. Dissolve FITC (1mg/ml, fluorescein isothiocyanate) in DMSO
4. Put 1ml dialysed antibody in a fresh tube
5. Add 30-50 ul FITC directly, very slowly, drop-by-drop using a Pipetteman
6. Incubate for 2 hrs at RT in the dark.
7. Dialyze against PBS (3 changes over 2 days) or put into gel filtration G25 fine column.
8. Store at 4C or -20C for long term storage
이런 방법이였습니다.
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