실험 Q&A Mol. Biol.-RNA > mRNA Analysis
in situ hybridization 이슈
레벨1 jiyoungsam (과기인)
Hi all Brix users!
I think it is better to type in english in terms of explaining what I am doing and what is going on with my in situ.
So I tried 3 times in total and all came out with no hybridization. I use S35 radioactive isotope to detect mRNA level on my rat brain slices.
My ultimate question is that i cannot figure out why the hybridization failed. When we look at the flim after the exposure, we could only see the whole tissue were transfered to the flim not specific to our target region of interest in the brain slices.. so big non-specific binding occured...
Is there anyone who could help me on what might have gone wrong during the process?
Thank you!
Bio마켓 프리미엄