실험 Q&A Mol. Biol.-DNA > PCR/RT-PCR/Q-PCR
real-time(CFX96) Melt curve로 Melt peak 그리기
레벨5 로소 (일반인)
Temperature | A3 |
55.00 | 12614.3 |
55.50 | 12550.9 |
56.00 | 12487.4 |
56.50 | 12424.0 |
57.00 | 12360.6 |
57.50 | 12297.2 |
58.00 | 12233.8 |
58.50 | 12167.6 |
59.00 | 12100.0 |
59.50 | 12030.5 |
60.00 | 11959.6 |
60.50 | 11887.1 |
61.00 | 11813.2 |
61.50 | 11737.3 |
62.00 | 11659.0 |
62.50 | 11577.6 |
63.00 | 11492.8 |
63.50 | 11403.7 |
64.00 | 11310.8 |
64.50 | 11213.9 |
65.00 | 11113.4 |
65.50 | 11009.7 |
66.00 | 10903.6 |
66.50 | 10795.3 |
67.00 | 10685.6 |
67.50 | 10574.5 |
68.00 | 10463.1 |
68.50 | 10351.2 |
69.00 | 10239.0 |
69.50 | 10126.4 |
70.00 | 10014.2 |
70.50 | 9901.4 |
71.00 | 9789.3 |
71.50 | 9677.5 |
72.00 | 9566.7 |
72.50 | 9456.7 |
73.00 | 9347.7 |
73.50 | 9238.8 |
74.00 | 9130.8 |
74.50 | 9023.1 |
75.00 | 8915.5 |
75.50 | 8808.8 |
76.00 | 8703.1 |
76.50 | 8597.9 |
77.00 | 8492.9 |
77.50 | 8388.7 |
78.00 | 8284.3 |
78.50 | 8179.7 |
79.00 | 8074.1 |
79.50 | 7966.2 |
80.00 | 7851.6 |
80.50 | 7722.2 |
81.00 | 7561.6 |
81.50 | 7348.4 |
82.00 | 7061.5 |
82.50 | 6691.1 |
83.00 | 6247.0 |
83.50 | 5762.6 |
84.00 | 5285.4 |
84.50 | 4866.3 |
85.00 | 4539.9 |
85.50 | 4315.6 |
86.00 | 4180.5 |
86.50 | 4110.3 |
87.00 | 4077.3 |
87.50 | 4060.7 |
88.00 | 4049.8 |
88.50 | 4040.5 |
89.00 | 4032.0 |
89.50 | 4023.4 |
90.00 | 4015.2 |
90.50 | 4006.9 |
91.00 | 3998.6 |
91.50 | 3990.4 |
92.00 | 3982.3 |
92.50 | 3974.1 |
93.00 | 3966.0 |
93.50 | 3958.2 |
94.00 | 3950.5 |
94.50 | 3942.8 |
95.00 | 3935.5 |
Temperature | A3 |
55.00 | 63.41 |
55.50 | 137.39 |
56.00 | 126.82 |
56.50 | 126.82 |
57.00 | 126.82 |
57.50 | 126.35 |
58.00 | 129.36 |
58.50 | 133.93 |
59.00 | 137.10 |
59.50 | 140.45 |
60.00 | 143.50 |
60.50 | 146.29 |
61.00 | 149.57 |
61.50 | 154.02 |
62.00 | 159.53 |
62.50 | 166.09 |
63.00 | 173.79 |
63.50 | 181.99 |
64.00 | 189.83 |
64.50 | 197.57 |
65.00 | 204.45 |
65.50 | 209.99 |
66.00 | 214.50 |
66.50 | 218.17 |
67.00 | 221.05 |
67.50 | 222.59 |
68.00 | 223.23 |
68.50 | 224.19 |
69.00 | 224.97 |
69.50 | 224.71 |
70.00 | 225.01 |
70.50 | 225.10 |
71.00 | 224.05 |
71.50 | 222.67 |
72.00 | 220.76 |
72.50 | 218.85 |
73.00 | 217.91 |
73.50 | 216.93 |
74.00 | 215.54 |
74.50 | 215.39 |
75.00 | 214.45 |
75.50 | 212.28 |
76.00 | 210.77 |
76.50 | 210.32 |
77.00 | 209.20 |
77.50 | 208.38 |
78.00 | 208.83 |
78.50 | 209.88 |
79.00 | 212.55 |
79.50 | 220.40 |
80.00 | 239.93 |
80.50 | 283.76 |
81.00 | 366.65 |
81.50 | 494.88 |
82.00 | 657.35 |
82.50 | 821.67 |
83.00 | 941.96 |
83.50 | 978.08 |
84.00 | 910.52 |
84.50 | 752.82 |
85.00 | 550.20 |
85.50 | 353.16 |
86.00 | 196.57 |
86.50 | 95.12 |
87.00 | 44.31 |
87.50 | 25.04 |
88.00 | 19.39 |
88.50 | 17.59 |
89.00 | 17.00 |
89.50 | 16.80 |
90.00 | 16.51 |
90.50 | 16.52 |
91.00 | 16.55 |
91.50 | 16.33 |
92.00 | 16.22 |
92.50 | 16.31 |
93.00 | 15.97 |
93.50 | 15.56 |
94.00 | 15.43 |
94.50 | 16.22 |
95.00 | 7.27 |
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