Lysis buffer의 조성
- Nonidet - P40(NP40) buffer
: 150mM sodium chloride 1.0% NP-40(Triton X-100 can be substituted for NP-40)
50mM Tris, pH 8.0
- RIPA buffer(Radio Immuno Precipitation As say buffer)
: 150mM sodium chloride, 1.0% NP-40 or Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS(sodium dodecycl sulfate), 50mM Tris pH 8.0
- Tris-HCl buffer
: 20mM Tris-HCl pH 7.5
- Tris-Triton buffer
: 10mM Tris pH7.4, 100mM NaCl, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 10% glycerol, 0.1% SDS, 0.5% deoxycholate
lysis buffer의 종류에 Nonidet - P40(NP40) buffer, RIPA buffer, Tris-HCl buffer, Tris-Triton buffer가 있는 건가요?
조성도 맞는지 봐주세요
알려주시면 정말 감사하겠습니다 |
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실험조아
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실전 실험 프로토콜 101
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