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Jackson lab에서 인용합니다.
Strain Description
Although C57BLKS/J is estimated to have more than 70% of its genome derived from C57BL/6J, these strains are phenotypically distinct. Diet-induced atherosclerotic lesions are much more severe in C57BLKS/J than in C57BL/6J or many other inbred strains. Compared with a panel of inbred strains, C57BLKS/J was found to have low total cholesterol and low HDL cholesterol when fed a normal diet and high total cholesterol and HDL cholesterol in an atherogenic diet. Paigen et al. found high levels of plasma bile salts in C57BLKS/J females after 8 weeks on an atherogenic diet. The mutations diabetes (Leprdb) and obese (Lepob) each express a much more severe phenotype on the C57BLKS/J background than on the C57BL/6J background. The Cpefat mutation causes severe obesity, hyperinsulinemia, and hyperglycemia on the C57BLKS/J background rather than the hyperinsulinemia, and mild obesity without hyperglycemia found on the HRS/J background. In a comparative study of 43 inbred strains, Barker and Peters found that C57BLKS/J had a relatively high percentage of reticulocytes per total number of erythrocytes, high white blood cell count per volume, with a high percentage of lymphocytes and basophils and a low percentage of neutrophils per total number of leukocytes. C57BLKS/J mice exhibit age related hearing loss by 3 months of age.
Strain Development
In October, 1947, while at The Sloan-kettering Institute, Dr. N. Kaliss obtained a C57BL/6J male from The Jackson Laboratory and obtained a female, which was reported to be one generation removed from the male, from a pen-bred colony of C57BL/6J of Dr. J. J. Biesele. Dr. Kaliss bred these together and maintained an inbreeding line of what he thought were C57BL/6J and brought them back with him to The Jackson Laboratory in 1948. He found that with continued inbreeding this strain rejected the C57BL/6-derived sarcoma E0771. It was subsequently determined that this strain had a genetic contamination untraced in origin. Rather than the H2b of C57BL/6J, this strain was found homozygous for the H2d haplotype found also in DBA/2J. Polymorphisms have been assessed to characterize this contamination and Mao et al., following on work of Naggert et al. and Slingsby et al., reported finding that the C57BLKS/J genome derived from 71% C57BL/6J, 25% DBA/2J, and 4% from a combination of C57BL/10J, a 129 source, and possibly some other undefined source. Clusters of 129-like alleles were found on Chr 4 and 15, C57BL/10-like alleles were found on Chr 11 and elsewhere in the genome, and additional unique alleles were also identified.
C57BL/KsJ
C57BL/Ks
C57BL/6J...
각각은 imbred mouse strain 혹은 substrain입니다.
어느곳에서 colony가 어떻게 유지되었는지에 따라 유전형이 다르게되고 따라서 다른 strain으로 만들어질 수 있는 것입니다.
원래 여러 stain의 origin은 Abbie Lathrop과 William Castle 에 의해 만들어진 것들이 많죠. 따라서 C57BL/6의 가계도나 Castle''s lineage를 찾아서 보시면 좋을 것 같습니다.
C57BL/6J와 C57BL/6
혹은 C57BL/KsJ와 C57BL/Ks
의 차이는 colony가 Jackson laboratory에서 유지되었는가 아닌가 입니다.
물론 다소 유전형의 차이가 있습니다.
db/db 혹은 db/m은 db allele의 genotype입니다.
db/db는 line 유지 중에 spontaneous mutation으로 생긴 mutant mouse line입니다. 이 allele이 db mutant homozygote이면 db/db이고 heterozygote이면 db/m입니다.
이에따라 obesity나 ...
중간에 답변이 짤려서 다시씁니다.
유전형이 db/db 혹은 db/m 이냐에 따라 당연히 표현형 즉 phenotype도 달라집니다.
db/db면 Ob/DM 이 잘 나타나겠지만 db/m이면 나타나지 않죠. 따라서 db/m은 control로 쓰일수 있을겁니다.
Mutant mouse study에서 기초적인 유전학적 지식은 필수라고 생각합니다.
질문하신 내용 정도는 숙지 하시고 실험하시기를 권합니다.