실험 Q&A Mol. Biol.-DNA > Gene Cloning
16S rDNA sequence 중에서 특이적 primer를 제작하려면
primer제작초보 (비회원)
universal primer를 이용해서 16S rDNA cloning을 해서 여러 미생물을 찾았는데요. 이런 미생물 중에서 어떤 한 종을 타겟으로 그것만 증폭하는 primer를 제작하려고 합니다. 예를 들어 NCBI assession no.AF425948(Rhodoferax ferrireducens 16S ribosomal RNA gene)인 미생물 sequence 중에서 특이적 primer를 제작하려면 어떻게 해야 하나요? 1 agagtttgat cctggctcag attgaacgct ggcggcatgc cttacacatg caagtcgaac 61 ggcagcacgg gagcaatcct ggtggcgagt ggcgaacggg tgagtaatat atcggaacgt 121 gcccagtcgt gggggataac gcagcgaaag ctgtgctaat accgcatacg atctctggat 181 gaaagcgggg gactcgcaag ggcctcgcgc gattggagcg gccgatatca gattagctag 241 ttggtggggt aaaagcccac caaggcgacg atctgtagct ggtctgagag gacgaccagc 301 cacactggaa ctgagacacg gtccagactc ctacgggagg cagcagtggg gaattttgga 361 caatgggcgc aagcctgatc caacaatgcc gcgtgcagga tgaaggcctt cgggttgtaa 421 actgcttttg tacggaacga aacggcctgc cctaatacgg tgggctaatg acggtaccgt 481 aagaataagc accggctaac tacgtgccag cagccgcggt aatacgtagg gtgcgagcgt 541 taatcggaat tactgggcgt aaagcgtgcg caggcggtta tataagacag atgtgaaatc 601 cccgggctca acctgggacc tgcatttgtg actgtatagc tagagtacgg tagaggggga 661 tggaattccg cgtgtagcag tgaaatgcgt agatatgcgg aggaacaccg atggcgaagg 721 caatcccctg gacctgtact gacgctcatg cacgaaagcg tggggagcaa acaggattag 781 ataccctggt agtccacgcc ctaaacgatg tcaactggtt gttgggtctt cactgactca 841 gtaacgaagc taacgcgtga agttgaccgc ctggggagta cggccgcaag gttgaaactc 901 aaaggaattg acggggaccc gcacaagcgg tggatgatgt ggtttaattc gatgcaacgc 961 gaaaaacctt acccaccttt gacatgtacg gaatccttta gagatagagg agtgctcgaa 1021 agagagccgt aacacaggtg ctgcatggct gtcgtcagct cgtgtcgtga gatgttgggt 1081 taagtcccgc aacgagcgca acccttgtca ttagttgcta catttagttg ggcactctaa 1141 tgagactgcc ggtgacaagc cggaggaagg tggggatgac gtcaagtcct catggccctt 1201 ataggtgggg ctacacacgt catacaatgg ctggtacaaa gggttgccaa cccgcgaggg 1261 ggagctaatc ccacaaagcc agtcgtagtc cggatcgtag tctgcaactc gactacgtga 1321 agtcggaatc gctagtaatc gtggatcaga atgtcacggt gaatacgttc ccgggtcttg 1381 tacacaccgc ccgtcacacc atgggagcgg gttctgccag aagtagttag cctaaccgca 1441 aggagggcga ttaccacggc agggttcgta 어디가 이 미생물의 특이적 부분인지 어떻게 알수 있을까요?
Bio마켓 프리미엄