BioLab
KIST Molecular & Cellular Neurobiology lab
권석규 교수
연구실 소개
연구실 홈페이지KIST 분자세포 신경생물학 실험실은 2018년 3월에 시작한 실험실로 서울 KIST 뇌과학연구소에 속해 있습니다. KIST는 1966년에 세워진 대한민국 최초의 정부출연연구소로서 반도체, 로봇, 의공학, 뇌과학, 에너지 등 다양한 분야의 종합 연구를 수행하고 있습니다. 저희 KIST 뇌과학연구소에는 신경회로, 인지 및 운동기능, 퇴행성 신경질환, 계산신경과학, 약물 개발, 마이크로 엔지니어링 등 다양한 배경을 가진 연구자분들이 모여 세계적으로 경쟁력있는 연구들을 진행하고 있습니다. 또한 미국, 일본, 캐나다 등 다양한 국적을 가진 Principal Investigator (PI)분들이 계셔서 자유로운 분위기의 연구환경을 가지고 있습니다.
연구분야
I. What are roles of neuronal organelles for synaptic function?
The basic unit of neuronal connectivity, the synapse, is the close apposition between the axonal presynaptic boutons and the postsynaptic dendrite of another neuron (Fig. 1 & 2). Neurotransmitter release is critically regulated by calcium level at presynaptic boutons, and postsynaptically, the temporal and spatial dynamics of calcium influx also plays critical roles in signal transduction and information processing. Therefore, identifying novel mechanisms regulating pre- and/or postsynaptic calcium dynamics in neurons will provide new insights into a wide range of important neurophysiological functions. Previous studies suggested that intracellular organelles like mitochondria and endoplasmic reticulum (ER) control calcium dynamics through their calcium uptake and release properties in various non-neuronal cell types. In addition, recent studies revealed that neuronal ER and mitochondria make contact and functionally interact by transferring calcium (Fig 2 & Video 1). However, their contribution to synapse development and function is still unknown. Our lab is investigating novel roles of intracellular organellar interaction and function for regulation of synapses.
II. Can neural circuit properties be determined by intracelluar oraganelles?
Our brain function is orchestrated by various types of neurons and their circuits. Researchers have been endeavoring to unveil mechanisms of neuronal connectivity based on structural and functional properties. However, contribution of subcellular components to neural circuit properties is still not well known. Our lab will expand the knowledge from in vitro studies to the circuit level using various techniques (Fig. 3-5).
Related publications
Hirabayashi Y*, Kwon SK*, Paek H, Pernice W, Paul MA, Lee J, Erfani P, Raczkowski A, Petrey DS, Liza A. Pon LA and Polleux F. (2017)
(* co-first author)
ER-mitochondria tethering by PDZD8 regulates Ca2+ dynamics in mammalian neurons
Science 358, 623-630 (2017)
Kwon SK, Sando R, Lewis TL, Hirabayashi Y, Maximov A and Polleux F. (2016)
PLoS Biology. 14(7):e1002516 (2016)
Lee A*, Hirabayashi Y*, Kwon SK*, Lewis TL* and Polleux F. (2018) (*co-first author)
Emerging roles of mitochondria in synaptic transmission and neurodegeneration
Curr. Op. in Physiol. (2018)
Kwon SK, Hirabayashi Y and Polleux F. (2016)
Organelle-specific sensors for monitoring Ca2+ dynamics in neurons
Front. Synaptic Neurosci. doi: 10.3389/fnsyn.2016.00029
연구성과
Research articles
20. Chunmei Jin, Hyojin Kang, Taesun Yoo, Jae Ryun Ryu, Ye-Eun Yoo, Ruiying Ma, Yinhua Zhang, Hyae Rim Kang, Yoonhee Kim, Hyunyoung Seong, Geul Bang, Sangwoo Park, Seok-Kyu Kwon, Woong Sun, Hyunkyung Kim, Jin Young Kim, Eunjoon Kim and Kihoon Han
The Neomycin Resistance Cassette in the Targeted Allele of Shank3B Knock-Out Mice Has Potential Off-Target Effects to Produce an Unusual Shank3 Isoform
Front. Mol. Neurosci., 2021 Jan 11;13:614435. doi: 10.3389/fnmol.2020.614435
19. Yinhua Zhang, Hyae Rim Kang, Seung-Hyun Lee, Yoonhee Kim, Ruiying Ma, Chunmei Jin, Ji-Eun Lim, Seoyeon Kim, Yeju Kang, Hyojin Kang, Su Yeon Kim, Seok-Kyu Kwon, Se-Young Choi and Kihoon Han
Enhanced Prefrontal Neuronal Activity and Social Dominance Behavior in Postnatal Forebrain Excitatory Neuron-Specific Cyfip2 Knock-Out Mice
Front. Mol. Neurosci., 2020 Oct 29;13:574947. doi: 10.3389/fnmol.2020.574947
18. Chan E Park, Youngbin Cho, In Cho, Hyunsu Jung, Byeongyeon Kim, Jennifer H. Shin, Sungyoung Choi, Seok-Kyu Kwon*, Young Ki Hahn*, and Jae-Byum Chang* (* co-corresponding author)
Super-Resolution Three-Dimensional Imaging of Actin Filaments in Cultured Cells and the Brain via Expansion Microscopy
ACS Nano 2020, 2020, 14, 11, 14999-15010. Publication Date:October 23, 2020
https://doi.org/10.1021/acsnano.0c04915
17. Gyu Hyun Kim, Yinhua Zhang, Hyae Rim Kang, Seung-Hyun Lee, Jiwon Shin, Chan Hee Lee, Hyojin Kang, Ruiying Ma, Chunmei Jin, Yoonhee Kim, Su Yeon Kim, Seok-Kyu Kwon, Se-Young Choi, Kea Joo Lee, Kihoon Han
Altered presynaptic function and number of mitochondria in the medial prefrontal cortex of adult Cyfip2 heterozygous mice
Mol Brain. 2020; 13: 123. Published online 2020 Sep 11. doi: 10.1186/s13041-020-00668-4
16. Kim H, Jung H, Jung H, Kwon SK, Ko J, Um JW.
The small GTPase ARF6 regulates GABAergic synapse development
Mol Brain. 2020 Jan 6;13(1):2. doi: 10.1186/s13041-019-0543-3.
15. Lewis TL*, Kwon SK*, Lee A, Shaw R, and Polleux F. (2018)
(* co-first author)
MFF-dependent mitochondrial fission regulates presynaptic release and axon branching by limiting axonal mitochondria size
Nature Communications 9, Article number: 5008 (2018)
14. Hirabayashi Y*, Kwon SK*, Paek H, Pernice W, Paul MA, Lee J, Erfani P, Raczkowski A, Petrey DS, Liza A. Pon LA and Polleux F. (2017)
(* co-first author)
ER-mitochondria tethering by PDZD8 regulates Ca2+ dynamics in mammalian neurons
Science 358, 623-630 (2017)
13. Bae YS, Yoon SH, Han JY, Woo J, Cho Y, Kwon SK, Bae YC, Kim D, Kim E and Kim MH. (2017)
Deficiency of aminopeptidase P1 causes behavioral hyperactivity, cognitive deficits, and hippocampal neurodegeneration
Genes, Brain, and Behavior. 2017 Aug 23. doi: 10.1111/gbb.12419
12. Lewis TL, Gergely FT, Kwon SK, Losonczy A and Polleux F. (2016)
Progressive decrease of mitochondrial motility during maturation of cortical axons in vitro and in vivo
Current Biology. 26(19):2602-2608 (2016)
11. Kwon SK, Sando R, Lewis TL, Hirabayashi Y, Maximov A and Polleux F. (2016)
LKB1 regulates mitochondria-dependent presynaptic calcium clearance and neurotransmitter release properties at excitatory synapses along cortical axons
PLoS Biology. 14(7):e1002516 (2016)
10. Choi Y, Nam J, Whitcomb DJ, Song YS, Kim D, Jeon S, Um JW, Lee SG, Woo J, Kwon SK, Li Y, Mah W, Kim HM, Ko J, Cho K and Kim E. (2016).
SALM5 trans-synaptically interacts with LAR-RPTPs in a splicing-dependent manner to regulate synapse development.
Scientific Reports 6: 26676 (2016)
9. Jang S, Oh D, Lee Y, Hosy E, Shin H, Riesen C, Whitcomb D, Warburton JM, Jo J, Kim D, Kim SG, Um SM, Kwon SK, Kim MH, Roh JD, Woo J, Jun H, Lee D, Mah W, Kim H, Kaang BK, Cho K, Rhee JS, Choquet D and Kim E. (2016)
Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity
Nature Neuroscience. 19(1):84-93
8. Baek ST, Copeland B, Yun EJ, Kwon SK, Guemez-Gamboa A, Schaffer AE, Kim S, Kang HC, Song S, Mathern GW and Gleeson JG. (2015)
An AKT3-FOXG1-reelin network underlies defective migration in human focal malformations of cortical development
Nature Medicine. 21:1445-1454
7. Woo J*, Kwon SK*, Nam JY*, Choi SW*, Takahashi H*, Krueger D, Park JH, Bae JY, Ko JW, Lee DM, Kim H, Kim MH, Bae YC, Chang SH, Craig AM and Kim E. (2013)
(* co-first author)
IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development.
Journal of Cell Biology, 201:929-944
6. Yoon KJ, Lee HR, Jo YS, An K, Jung SY, Jeong MW, Kwon SK, Kim NS, Jeong HW, Ahn SH, Kim KT, Lee K, Kim E, Kim JH, Choi JS, Kaang BK and Kong YY. (2012)
Mind bomb-1 is an essential modulator of long-term memory and synaptic plasticity via the Notch signaling pathway.
Molecular Brain 5:40
5. Kwon SK*, Woo J*, Kim SY, Kim H and Kim E. (2010). (* co-first author)
Trans-synaptic adhesions between netrin-G ligand-3 (NGL-3) and receptor tyrosine phosphatases LAR, PTPδ, and PTPσ via specific domains regulate excitatory synapse formation.
J. Biol. Chem. 285:13966-13978
4. Lim SH, Kwon SK, Lee MK, Moon J, Jeong DG, Park E, Kim SJ, Park BC, Rhu SE, Yu DY, Kim E, Myung PK, and Lee JR. (2009).
Regulation of synapse formation by PTPRT through interaction with cell adhesion molecules and Fyn.
The EMBO Journal 28: 3564 - 3578
3. Woo J*, Kwon SK*, Choi S, Kim S, Lee JR, Dunah AW, Sheng M and Kim E. (2009).
(*co-first author)
Trans-synaptic adhesion between NGL-3 and LAR regulates the formation of excitatory synapses.
Nature Neuroscience 12:428-437.
2. Kim Y, Park J, Kim S, Song S, Kwon SK, Lee SH, Kitada T, Kim JM and Chung J. (2008)
PINK1 controls mitochondrial localization of Parkin through direct phosphorylation.
Biochem Biophys Res Commun. 377(3):975-80.
1. Kim S, Burette A, Chung HS, Kwon SK, Woo J, Lee HW, Kim K, Kim H, Weinberg RJ and Kim E. (2006).
NGL family PSD-95-interacting adhesion molecules regulate excitatory synapse formation.
Nature Neuroscience 9:1294-1231.
Review articles
6. Hyunsu Jung, Su Yeon Kim, Fatma Sema Canbakis Cecen, Yongcheol Cho, and Seok-Kyu Kwon*
Dysfunction of mitochondrial Ca2+ regulatory machineries in brain aging and neurodegenerative diseases
Frontiers in Cell and Developmental Biology, 2020; 8: 599792.
doi: 10.3389/fcell.2020.599792
5. Rangaraju V, Lewis TL Jr, Hirabayashi Y, Bergami M, Motori E, Cartoni R, Kwon SK*, Courchet J*. (2019) (* co-corresponding author)
Pleiotropic Mitochondria: The Influence of Mitochondria on Neuronal Development and Disease
J. Neuroscience. 2019 Oct 16;39(42):8200-8208
4. Lee A*, Hirabayashi Y*, Kwon SK*, Lewis TL* and Polleux F. (2018) (*co-first author) Emerging roles of mitochondria in synaptic transmission and neurodegeneration
Curr. Op. in Physiol. (2018)
3. Lee A*, Hirabayashi Y*, Kwon SK*, Lewis TL* and Polleux F. (2018) (*co-first author) Emerging roles of mitochondria in synaptic transmission and neurodegeneration
Curr. Op. in Physiol. (2018)
2. Kwon SK, Hirabayashi Y and Polleux F. (2016)
Organelle-specific sensors for monitoring Ca2+ dynamics in neurons
Front. Synaptic Neurosci. doi: 10.3389/fnsyn.2016.00029
1. Woo J*, Kwon SK* and Kim E. (2009). (* co-first author).
The NGL family of leucine-rich repeat-containing synaptic adhesion molecules.
Mol. Cell. Neuroscience 42:1-10.
연구실 구성원
지도교수: 권석규
박사과정: 김수연, Fatma Sema Canbakis Cecen
석사과정: 정현수, 김원석
인턴: 김강연, 배혜연
Contact :skkwon@kist.re.kr
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