CRISPR in Animal Model Generation - Functionalizing Genome Editing
for a Broad Range of Targets
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You will learn:
Discuss best practices for creating Knockin cell lines.
Large insertions can be achieved with good efficiencies via direct embryo injection and ssDNA.
NGS analysis enables detection and quantification of CRISPR editing outcomes on a diverse range of sample inputs including cell pools, clonal populations, and animal tissues.
CRIS.py is a user friendly NGS analysis pipeline that is open source.
NGS can be used to genotype both small and large insertion events.
Related products and services:
GenCRISPR™ Mammalian Cell Line Services
CRISPR Synthetic Guide RNA Services
Single-Stranded DNA Synthesis Service
GenCRISPR™ gRNA/Cas9 Plasmids