Discuss best practices for creating Knockin cell lines.
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Large insertions can be achieved with good efficiencies via direct embryo injection and ssDNA.
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NGS analysis enables detection and quantification of CRISPR editing outcomes on a diverse range of sample inputs including cell pools, clonal populations, and animal tissues.
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CRIS.py is a user friendly NGS analysis pipeline that is open source.
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NGS can be used to genotype both small and large insertion events.