Next Generation sequencing based spatial gene expression profiling solution
Spatial characterization of the immune contexture in the tumor microenvironment (TME) is essential for understanding the relationship between immune and tumor cells. However, it has proven difficult to perform such studies in a highly multiplexed manner using limited samples. To address this unmet need, we have developed an imaging and tissue-sampling platform designed to simultaneously analyze up to hundreds of tumor and immune proteins in distinct locations on a single FFPE tissue section. To supplement this capacity, we have recently developed an RNA detection chemistry that can profile thousands of transcripts using NGS sequencing platforms on the same FFPE sample types. Using this novel technology, called Digital Spatial Profiling (DSP), we demonstrate multiplexed protein and RNA analysis of defined regions of interest within a tumor enabling systematic interrogation of the immune status in the tumor microenvironment.