한빛사 논문
Yoon-Tae Kanga, Emma Purcella, Colin Palacios-Rolstona, Ting-Wen Loa, Nithya Ramnathb, Shruti Jollyc, and Sunitha Nagratha,*
aDepartment of Chemical Engineering and Biointerface Institute, University of Michigan, 2800 Plymouth Road, NCRC B10-A184, Ann Arbor, MI 48109, USA
bDepartment of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA
cDepartment of Radiation Oncology, Michigan Medicine, University of Michigan, 1500 E Medical Center Dr., Ann Arbor, MI 48109, USA
*To whom correspondence should be addressed.
Abstract
Extracellular vesicles (EVs) are emerging as a potential diagnostic test for cancer. Owing to the recent advances in microfluidics, on‐chip EV isolation is showing promise with respect to improved recovery rates, smaller necessary sample volumes, and shorter processing times than ultracentrifugation. Immunoaffinity‐based microfluidic EV isolation using anti‐CD63 is widely used; however, anti‐CD63 is not specific to cancer‐EVs, and some cancers secrete EVs with low expression of CD63. Alternatively, phosphatidylserine (PS), usually expressed in the inner leaflet of the lipid bilayer of the cells, is shown to be expressed on the outer surface of cancer‐associated EVs. A new exosome isolation microfluidic device (newExoChip), conjugated with a PS‐specific protein, to isolate cancer‐associated exosomes from plasma, is presented. The device achieves 90% capture efficiency for cancer cell exosomes compared to 38% for healthy exosomes and isolates 35% more A549‐derived exosomes than an anti‐CD63‐conjugated device. Immobilized exosomes are then easily released using Ca 2+ chelation. The recovered exosomes from clinical samples are characterized by electron microscopy and western‐blot analysis, revealing exosomal shapes and exosomal protein expressions. The newExoChip facilitates the isolation of a specific subset of exosomes, allowing the exploration of the undiscovered roles of exosomes in cancer progression and metastasis.
Keywords : cancer‐associated exosomes, exosome isolation, extracellular vesicles, lung cancer, melanoma, microfluidics
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