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BioLab
BioLab
Univ. of Southern California, Ophthalmology and Physiology and Neuroscience, Lee Letina Lab.
이선영 교수
- 등록2025.06.23
- 조회956
연구실 소개
연구실 홈페이지At the Lee Retina Lab, our dedicated researchers are passionate about saving and restoring vision from retinal diseases such as dry age-related macular degeneration (AMD), wet AMD, diabetic retinopathy, and inherited retinal diseases. We are utilizing stem cell derived EV and bioengineered EV to understand the pathophysiology of retinal diseases and develop intraocular therapeutics. We are also using the various cutting edge multitopic strategies including non-coding RNA, proteomics and lipidomic to study EVs.
Sun Young Lee, MD, Ph.D. (이선영)
Associate Professor of Ophthalmology and Physiology and Neuroscience with Tenure, Keck School of Medicine of USC
Education and Training
1999 MD, Pusan National University, S. Korea
2007 PhD, University of Ulsan, S. Korea
2007 Ophthalmology Residency/Vitreoretinal Surgery Fellowship, Asan Medical Center, S. Korea
2010 Post-doctoral Fellowship, Johns Hopkins University, MD, USA
2016 Ophthalmology Residency, University of Southern California, CA, USA
2018 Vitreoretinal Surgery Fellowship, University of Iowa, IA, USA
연구분야
Comprehensive Characterization of Extracellular Vesicles
Extracellular vesicles from human embryonic stem cell-derived RPE cells were recovered using microfluidic tangential flow filtration and differential ultracentrifugation. We characterized the EVs using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), their tetraspanin expression, and multi-surface epitope heterogeneity.
Retinal Uptake of EVs
Live retinal imaging performed at 3 and 24 h and retina flat-mounts obtained 1 day and 1 week after intravitreal injection of tagged exosomes (orange) show retinal penetration and distribution of EVs.
Retinal sections obtained 1 day after intravitreal injection of tagged EVs (ret dots with yellow circles) show that EVs were taken up by both the inner and outer retina.
Retinal sections obtained 3 h after systemic injection of tagged EVs (ret dots with yellow circles) via tail vein injection show that EVs were taken up by the retina, suggesting that EVs can cross the blood-retina barrier (BRB).
ASL Engineered Exosomes
CNV in a laser-induced mouse model is confirmed using color fundus photography, fluorescein angiography, OCT, and H&E histology.
Integrin 𝛼V expression in CNV lesions is significantly higher than in healthy retina.
RGD is one of the major binding ligands for integrin families.
ASL-modified EVs, composed of a membrane Anchor (BODIPY - green), Spacer (PEG), and targeting Ligand [cyclic Arg-Gly-Asp (RGD) peptide] show an almost 90% colocalization with integrin 𝛼V (red)
ASL Engineered Exosomes loaded with miR
Clustering of our ASL-modified, miR-loaded EVs showed 4 distinctive clusters: ASL-positive EVs, miR-positive EVs, double-positive EVs, and double-negative EVs.
연구성과
최근 5년
Lee SY, Klingeborn M, Bulte JWM, Chiu DT, Chopp M, Cutler CW, Das S, Egwuagu CE, Fowler CD, Hamm-Alvarez SF, Lee H, Liu Y, Mead B, Moore TL, Ravindran S, Shetty AK, Skog J, Witwer KW, Djalilian AR, Weaver AM. A perspective from the National Eye Institute Extracellular Vesicle Workshop: Gaps, needs, and opportunities for studies of extracellular vesicles in vision research. J Extracell Vesicles. 2024 Dec;13(12):e70023. doi: 10.1002/jev2.70023. PMID: 39665315; PMCID: PMC11635481.
Pollalis D (#), Nair GKG (#), Leung J (^), Bloemhof CM (+), Bailey JK, Pennington BO, Kelly KR, Khan AI, Yeh AK, Sundaram KS, Clegg DO, Peng CC, Xu L, Georgescu C, Wren JD, Lee SY. Dynamics of microRNA secreted via extracellular vesicles during the maturation of embryonic stem cell-derived retinal pigment epithelium. J Extracell Biol. 2024 Sep 13;3(9):e70001. doi: 10.1002/jex2.70001. PMID: 39281021; PMCID: PMC11393772.
Dhodapkar RM (*), Jung E (*), Lee SY. An Eye on Extracellular Vesicles: Trends and Clinical Translations in Vision Research. Ophthalmol Sci. 2024 Sep 12;5(1):100619. doi: 10.1016/j.xops.2024.100619. PMID: 39584184; PMCID: PMC11585720.
Beatson B (*), Lee SY. Preretinal Deposits in a Keratoprosthesis-Associated Bacterial Endophthalmitis. Ophthalmol Retina. 2024 Nov;8(11):e42. doi: 10.1016/j.oret.2024.04.004. Epub 2024 May 11. PMID: 38739068.
Leung J (^), Pollalis D (#), Nair GKG (#), Bailey JK, Pennington BO, Khan AI, Kelly KR, Yeh AK, Sundaram KS, Clegg DO, Peng CC, Xu L, Lee SY. Isolation and Characterization of Extracellular Vesicles Through Orthogonal Approaches for the Development of Intraocular EV Therapy. Invest Ophthalmol Vis Sci. 2024 Mar 5;65(3):6. doi: 10.1167/iovs.65.3.6. PMID: 38466285; PMCID: PMC10929743.
Pollalis D (#), Calle AG, Martinez-Camarillo JC, Ahluwalia K, Hinman C, Mitra D, Lebkowski J, Lee SY, Thomas BB, Ahmed F, Chan V, Junge JA, Fraser S, Louie S, Humayun M. Scaling up polarized RPE cell supernatant production on parylene membrane. Exp Eye Res. 2024 Mar;240:109789. doi: 10.1016/j.exer.2024.109789. Epub 2024 Jan 17. PMID: 38242423.
Thenappan A(*), Nanda A(+), Lee CS (*), Lee SY. Retinitis Pigmentosa Masquerades: Case Series and Review of the Literature. J Clin Med. 2023 Aug 28;12(17):5620. doi: 10.3390/jcm12175620. PMID: 37685687.
Ahluwalia K, Martinez-Camarillo JC, Thomas BB, Naik A, Gonzalez-Calle A, Pollalis D (#), Lebkowski J, Lee SY, Mitra D, Louie SG, Humayun MS. Polarized RPE Secretome Preserves Photoreceptors in Retinal Dystrophic RCS Rats. Cells. 2023 Jun 22;12(13):1689. doi: 10.3390/cells12131689. PMID: 37443724; PMCID: PMC10340490.
Pollalis D (#), Nanda AV (+), Nair GKG (#), Lee SY. Fiji-Assisted Automatic Quantitative Volumetric Analysis of Choroidal Neovascularization in a Laser-Induced Choroidal Neovascularization Mouse Model. Transl Vis Sci Technol. 2023 Apr 3;12(4):10. doi: 10.1167/tvst.12.4.10. PMID: 37043336; PMCID: PMC10103716.
Humayun MS, Lee SY. Advanced Retina Implants. Ophthalmol Retina. 2022 Oct;6(10):899-905. doi: 10.1016/j.oret.2022.04.009. Epub 2022 Apr 15. PubMed PMID: 35436597.
McClard CK (*), Pollalis D (#), Jamshidi F (*), Kingsley R, Lee SY. Utility of No-Charge Panel Genetic Testing for Inherited Retinal Diseases in a Real-World Clinical Setting. J Vitreoretin Dis. 2022 Sep-Oct;6(5):351-357. doi: 10.1177/24741264221100936. Epub 2022 Aug 25. PubMed PMID: 36213299; PubMed Central PMCID: PMC9542391.
Pollalis D (#), Kim D, Nair GKG (#), Kang C (#), Nanda AV (+), Lee SY. Intraocular RGD-Engineered Exosomes and Active Targeting of Choroidal Neovascularization (CNV). Cells. 2022 Aug 18;11(16). doi: 10.3390/cells11162573. PubMed PMID: 36010651; PubMed Central PMCID: PMC9406786.
Nair GKG (#), Pollalis D (#), Wren JD, Georgescu C, Sjoelund V, Lee SY. Proteomic Insight into the Role of Exosomes in Proliferative Vitreoretinopathy Development. J Clin Med. 2022 May 11;11(10). doi: 10.3390/jcm11102716. PubMed PMID: 35628842; PubMed Central PMCID: PMC9143131.
LaGrow AL (+), Schatzman SN (+), Amayem OA (+), Bradford RH, Shah VA, Chen S, Lee SY. Endophthalmitis after transconjunctival pars plana vitrectomy: a 6-year experience without prophylactic intraoperative subconjunctival antibiotics. Retina. 2021 Mar 1;41(3):531-537. doi: 10.1097/IAE.0000000000002905. PubMed PMID: 32826794; PubMed Central PMCID: PMC7889285.
연구실 구성원
PI : Sun Young Lee
Post-Doctoral Fellow : Dimitri Pollalis, Mahrou Sadri
Research Associate: Tongzhou Xu
Medical Student Research Assistant : Clarisa Marie Bloemhof, Susan Kishi, Miso Ko
Research Assistant: Justin Leung
Undergraduate Research Assistant : Amy Li
Homepage : https://www.leeretinalab.com/
Congtact : SunYoung.lee@med.usc.edu
하고싶은 이야기
We welcome applications from passionate and motivated individuals at all levels, including visiting scholars, graduate students, and postdoctoral fellows. If you're interested in joining our team, please send your CV, cover letter, and contact information for three references.
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