Tien V Vu (경상국립대학교) | 한빛사논문 > 한빛사

한빛사논문

조회121

Tien V VuTien Van Vu

경상국립대학교

CV File 471 kb

Nat. Commun., 02 December 2025;16,10428(2025) | https://doi.org/10.1038/s41467-025-66715-7 The evolving landscape of precise DNA insertion in plants

Tien Van Vu^1,4, Ngan Thi Nguyen^1,4, Jihae Kim¹, Yeon Woo Sung¹, Woo Sik Chung¹ & Jae-Yean Kim^1,2,3

¹Division of Applied Life Science (BK21 Four Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Republic of Korea.
²Division of Life Science, Gyeongsang National University, Jinju, Republic of Korea.
³Nulla Bio Inc., Jinju, Republic of Korea.
⁴These authors contributed equally: Tien Van Vu, Ngan Thi Nguyen.

Corresponding authors
Correspondence to Tien Van Vu or Jae-Yean Kim.

Abstract

Precise DNA insertion into plant genomes is central to advancing crop improvement and synthetic biology. CRISPR-Cas systems have enabled programmable DNA integration using tools such as gene targeting (GT), prime editing (PE), and recombinase- or transposase-based platforms. These tools are transitioned from theoretical concepts to practical applications, supporting applications like in-locus protein tagging, regulatory element engineering, and multi-gene stacking. Key challenges persist, such as inefficient large-fragment insertion, delivery barriers, and regulatory hurdles. This review traces the evolution from random to CRISPR-Cas-based systems, analyzes current limitations, and discusses emerging solutions paving the way for predictable DNA insertion in modern plant biotechnology.

논문정보

형식Review Paper
게재일2025년 12월 (BRIC 등록일 2025-12-03)
연구진국내 연구진
분야 생명과학 > 식물학

댓글 작성 로그인

CV 열람하기

연락처 보기