이은영 (가톨릭대학교) | 한빛사논문 > 한빛사

한빛사논문

조회105

가톨릭대학교

CV File

Mater. Today Bio, Volume 35, December 2025, 102591 | https://doi.org/10.1016/j.mtbio.2025.102591 Development of a three-dimensional airway organoid-pillar dish (3D AO-PD) platform for particulate matter-induced airway toxicity analysis

Eunyoung Lee ^a,b,1, Sang-Yun Lee ^c,1, Yu-Jeong Seong ^a,b, Jeong Uk Lim ^d, Jae-Young Jeong ^e, Ji-O Ryu ^e, Yongki Hwang ^a,b, Hyobin Won ^a,b, Seok Whan Moon ^f, Young Du Kim ^f, Kyung Soo Kim ^f, Mi Hyoung Moon^f, Seung Keun Yoon ^f, Yeoun Eun Sung ^g, Meejeong Kim^g, Bosung Ku ^h, Dong Woo Lee^e

^aDivision of Pulmonology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea
^bPostech-Catholic Biomedical Engineering Institute, Songeui Multiplex Hall, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea
^cGraduate School of New Drug Discovery and Development, Chungnam National University, Daejeon, 34134, Republic of Korea
^dDivision of Pulmonary, Critical Care and Allergy, Department of Internal Medicine, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea
^eDepartment of Biomedical Engineering, Gachon University, Seongnam, 13120, Republic of Korea
^fDepartment of Thoracic and Cardiovascular Surgery, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea
^gDepartment of Hospital Pathology, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea
^hCentral R & D Center, Medical & Bio Decision (MBD) Co., Ltd, Suwon, 16229, Republic of Korea

¹These authors contributed equally to this work: Eunyoung Lee and Sang-Yun Lee.
Corresponding author: Dong Woo Lee

Abstract

Particulate matter (PM) is a major component of air pollution and is associated with respiratory diseases and lung dysfunction. Physiologically relevant models are essential for studying PM-induced airway responses in vitro. Conventional two-dimensional (2D) monolayer models comprising only bronchial epithelial cells are limited in their ability to mimic the complex structure of airway tissue. In contrast, three-dimensional (3D) airway organoids contain basal, ciliated, and goblet cells, which better reflect airway architecture. However, the standardization of 3D cultures has been a major challenge. In the present study, to address this, we developed the 3D Airway Organoid-Pillar Dish (AO-PD) model, a platform that enables reproducible formation of standardized 3D airway. We compared this model with a 2D monolayer model under PM exposure. The 3D AO-PD model showed higher cell viability and significant inflammatory and epithelial–mesenchymal transition responses than that of the 2D model. These results indicate that the 3D AO-PD model more accurately reflects PM-induced airway damage and provides greater resistance to environmental insults owing to its pseudostratified epithelial structure and enhanced cell–cell interactions. Thus, the 3D AO-PD model serves as a robust in vitro platform for investigating PM-induced airway toxicity and inflammation-related responses.

논문정보

형식Research article
게재일2025년 12월 (BRIC 등록일 2025-12-02)
연구진국내 연구진
분야 바이오·의료융합 > 바이오·의료융합 기타

이은영 님 전체 논문보기

연구자 ID

댓글 작성 로그인

CV 열람하기

연락처 보기