한빛사
>
한빛사논문
한빛사논문
- 조회164
이희영Heeyoung Lee
KAIST
Nat. Commun., 31 October 2025;16,9637(2025) | https://doi.org/10.1038/s41467-025-64635-0
A Rho GTPase-effector ensemble governs cell migration behavior
Heeyoung Lee1,11, Sangkyu Lee2,11, Yeji Seo3,11, Dongsan Kim4,11, Yohan Oh5,6, Juae Jin1, Bobae Hyeon1, Younghyun Han4, Hyunjun Kim7, Yong Jin Lee3, Ho Min Kim1, Gabsang Lee8, Kwang-Hyun Cho4 & Won Do Heo1,9,10
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
2Center for Cognition and Sociality, Institute for Basic Science, Daejeon, Republic of Korea.
3Department of R&D, Hulux, Seongnam, Republic of Korea.
4Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology(KAIST), Daejeon, Republic of Korea.
5Department of Biomedical Science, Graduate School of Biomedical Science & Engineering, Hanyang University, Seoul, Republic of Korea.
6Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul, Republic of Korea.
7Department of Biology, Johns Hopkins University, Baltimore, MD, USA.
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
2Center for Cognition and Sociality, Institute for Basic Science, Daejeon, Republic of Korea.
3Department of R&D, Hulux, Seongnam, Republic of Korea.
4Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology(KAIST), Daejeon, Republic of Korea.
5Department of Biomedical Science, Graduate School of Biomedical Science & Engineering, Hanyang University, Seoul, Republic of Korea.
6Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul, Republic of Korea.
7Department of Biology, Johns Hopkins University, Baltimore, MD, USA.
8Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
9Department of Cognitive Brain Science, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
10KAIST Institute for the BioInnovation (KIBI), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
11These authors contributed equally: Heeyoung Lee, Sangkyu Lee, Yeji Seo, Dongsan Kim.
Corresponding authors
Correspondence to Gabsang Lee, Kwang-Hyun Cho or Won Do Heo.
9Department of Cognitive Brain Science, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
10KAIST Institute for the BioInnovation (KIBI), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
11These authors contributed equally: Heeyoung Lee, Sangkyu Lee, Yeji Seo, Dongsan Kim.
Corresponding authors
Correspondence to Gabsang Lee, Kwang-Hyun Cho or Won Do Heo.
Abstract
How can a cell navigate its environment without any external cues? Since such cues are not always present in the environment, cells rely on internal machinery to explore their surroundings. Although Rho GTPases are known for orchestrating cell motility, the intrinsic Rho GTPase-effector mechanisms governing spontaneous migration remain incompletely understood. Here we show an imaging-based method that profiles protein-protein interactions (PPIs) through phase-separated condensates. By applying this method to hundreds of interaction profiles between Rho small GTPases and their effector proteins, we uncovered two intrinsic mechanisms governing cell migration. Formin-like protein (FMNL) determines the front of the cell by restricting Cdc42 activity, establishing front-rear polarity. In contrast, Rac1-ROCK-interaction-mediated arc stress fiber formation at the front inherently enables spontaneous directional changes and enhances cellular responses to external cues. Our findings elucidate the intricate roles of the Rho GTPase-effector ensemble that governs cell migration behavior, revealing an intrinsic program for efficient motility strategies.
논문정보
- Research article
- 2025년 10월 (BRIC 등록일 2025-11-03)
- 국내(교신)+국외 연구진
- 생명과학 > 세포생물학
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
관련분야 논문보기
