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Adv. Sci., 25 September 2025 | https://doi.org/10.1002/advs.202511889
Bioengineered Skin Grafts from Patient-Derived Decellularized Extracellular Matrix and Autologous Cells for Personalized Regenerative
RaeHui Kang 1, Suyeon Shin 2, Yurim Choi 2, WonJun Jang 3 4, Soojin Park 3 4, Mi Kyung Lee 5, Hyun-Jong Cho 6, Yu Shrike Zhang 7, Han-Jun Kim 3 8 *, Bo Young Park 5 *, Junmin Lee 1 2 *
1Division of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 37666, Republic of Korea.
2Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37666, Republic of Korea.
3Interdisciplinary Major Program in Innovative Pharmaceutical Sciences, Korea University, Sejong, 30019, Republic of Korea.
4College of Pharmacy, Korea University, Sejong, 30019, Republic of Korea.
5Department of Plastic and Reconstructive Surgery, School of Medicine, Ewha Womans University, Seoul, 03760, Republic of Korea.
6Department of Pharmacy, College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of Korea.
7Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.
8Department of Plastic and Reconstructive Surgery, College of Medicine, Korea University, Seoul, 02841, Republic of Korea.
2Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37666, Republic of Korea.
3Interdisciplinary Major Program in Innovative Pharmaceutical Sciences, Korea University, Sejong, 30019, Republic of Korea.
4College of Pharmacy, Korea University, Sejong, 30019, Republic of Korea.
5Department of Plastic and Reconstructive Surgery, School of Medicine, Ewha Womans University, Seoul, 03760, Republic of Korea.
6Department of Pharmacy, College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of Korea.
7Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.
8Department of Plastic and Reconstructive Surgery, College of Medicine, Korea University, Seoul, 02841, Republic of Korea.
*Corresponding authors: correspondence to Han-Jun Kim, Bo Young Park or Junmin Lee
Abstract
The skin, as the body's largest organ, plays vital protective and regulatory roles, making it a key target in regenerative medicine. However, current skin models often lack patient specificity and fail to recapitulate native extracellular matrix (ECM) composition, limiting their clinical relevance. This study presents a 3D-bioprinted skin model using a patient-derived decellularized ECM (pddECM) bioink combined with keratin-alginate (KA) bioink, mimicking native skin architecture and function. The pddECM supports high viability of human dermal fibroblasts (HDFs), promoting collagen I production and robust ECM remodeling, while the KA bioink enhances basal keratinocyte activation and cornification. The construct exhibits improved cell migration and angiogenesis, contributing to effective tissue integration and reduced hypoxic stress. Cytokine profiling reveals upregulation of ICAM-1 and complement C5, which are associated with enhanced keratinocyte motility and rapid matrix remodeling, while downregulation of pro-inflammatory cytokines (IL-4 and IL-8) suggests a favorable, fibrosis-suppressive environment. In vivo, GelMA and GelMA+pddECM scaffolds accelerated wound closure without local or systemic toxicity, preserving dermal thickness and inducing migrating epidermal tongue (MET) expression. This patient-specific, bioactive skin model holds strong potential as a next-generation platform for personalized wound healing, drug screening, and high-fidelity skin grafting in translational tissue engineering.
논문정보
- Research article
- 2025년 09월 (BRIC 등록일 2025-09-26)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
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