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Adv. Healthc. Mater., Jul 02 2025, | https://doi.org/10.1002/adhm.202501882
Ultra-Tiny Gelatin Nanoparticles-Assisted 3D Stem Cell Spheroids for Engineering Tissue Regeneration
Dream Kim 1,2,3, Woochan Kim 1,2,3, Harshita Sharma 1,2,3, Shinyull Lee 1,2,3, Chaeyeon Park 1,2,3, Sunho Park 4, Chan Yeong Heo 5,6, Jangho Kim 1,2,3
1Department of Convergence Biosystems Engineering, Chonnam National University, Gwangju, 61186, Republic of Korea.
2Department of Rural and Biosystems Engineering, Chonnam National University, Gwangju, 61186, Republic of Korea.
3Interdisciplinary Program in IT-Bio Convergence System, Chonnam National University, Gwangju, 61186, Republic of Korea.
4Department of Bio-Industrial Machinery Engineering, Pusan National University, Miryang-si, 50463, Republic of Korea.
5Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam, 13620, Republic of Korea.
6Department of Plastic and Reconstructive Surgery, College of Medicine, Seoul National University, Seoul, 03080, Republic of Korea.
D.K. and W.K. contributed equally to this work.
Corresponding Author : Jangho Kim
1Department of Convergence Biosystems Engineering, Chonnam National University, Gwangju, 61186, Republic of Korea.
2Department of Rural and Biosystems Engineering, Chonnam National University, Gwangju, 61186, Republic of Korea.
3Interdisciplinary Program in IT-Bio Convergence System, Chonnam National University, Gwangju, 61186, Republic of Korea.
4Department of Bio-Industrial Machinery Engineering, Pusan National University, Miryang-si, 50463, Republic of Korea.
5Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam, 13620, Republic of Korea.
6Department of Plastic and Reconstructive Surgery, College of Medicine, Seoul National University, Seoul, 03080, Republic of Korea.
D.K. and W.K. contributed equally to this work.
Corresponding Author : Jangho Kim
Abstract
Stem cell spheroids offer an effective approach for tissue repair, disease modeling, and therapeutic applications. However, traditional spheroid cultures face certain limitations, such as uncontrolled cell proliferation and size, lack of structural complexity, and limited nutrient diffusion, eventually causing core apoptosis. Therefore, this study aims to propose an ultra-tiny gelatin nanoparticle (GNP)-assisted spheroid culture system designed to promote compact spheroid formation and enhance intra-/intercellular functionality. Highly biocompatible GNPs (80-150 nm) are fabricated with unique properties that promote cell-cell and cell-extracellular matrix (ECM) interactions, thereby enhancing functionality in stem cell spheroids. Compared to traditional stem cell spheroid culture methods, GNP-stem cell spheroids show improved cell viability, proliferation, and cellular complexity. Furthermore, the time-dependent regenerative potential of fabricated GNP-stem cell spheroids is investigated in hard and soft tissue defect sites. These findings indicate the significant role of GNP-stem cell spheroids in accelerating bone and skin tissue regeneration. These groundbreaking findings establish GNP-enhanced spheroids as a transformative innovation in regenerative medicine, surpassing traditional 3D stem cell therapies and advancing tissue engineering and biomedical research.
논문정보
- Research article
- 2025년 07월 (BRIC 등록일 2025-07-04)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
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