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Biomater. Res., 2 Jul 2025, 29 0226 | https://doi.org/10.34133/bmr.0226
Biomimetic Catechol-Incorporated Polyacrylonitrile Nanofiber Scaffolds for Tissue Engineering of Functional Salivary Glands
Seokjun Kwon1†, Ji Hyun Ryu2†, Junchul Kim1†, Hyun Ho Shin2, Gehoon Chung1, Ali Taghizadeh3, Jung-Hwan Lee3, Jongho Kim4, Bon-Cheol Ku5, Kyungpyo Park1*, and Sang-woo Lee1,6*
1Department of Physiology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul,Republic of Korea.
2Department of Biomedical Materials Science, Graduate School of JABA, Department ofCarbon Convergence Engineering, Department of Chemical Engineering, Smart Convergence Materials Analysis Center, Wonkwang University, Iksan, Jeonbuk, Republic of Korea.
3Institute of Tissue RegenerationEngineering (ITREN), Dankook University, Cheonan, Chungcheongnam-do, Republic of Korea.
4Departmentof Textile System Engineering, Kyungpook National University, Daegu, Republic of Korea.
5Institute ofAdvanced Composite Materials, Korea Institute of Science and Technology (KIST), Wanju, Republic of Korea.
6Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, Republic of Korea.
*Address correspondence to: K.P.; S.-w.L.
†These authors contributed equally to this work.
1Department of Physiology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul,Republic of Korea.
2Department of Biomedical Materials Science, Graduate School of JABA, Department ofCarbon Convergence Engineering, Department of Chemical Engineering, Smart Convergence Materials Analysis Center, Wonkwang University, Iksan, Jeonbuk, Republic of Korea.
3Institute of Tissue RegenerationEngineering (ITREN), Dankook University, Cheonan, Chungcheongnam-do, Republic of Korea.
4Departmentof Textile System Engineering, Kyungpook National University, Daegu, Republic of Korea.
5Institute ofAdvanced Composite Materials, Korea Institute of Science and Technology (KIST), Wanju, Republic of Korea.
6Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, Republic of Korea.
*Address correspondence to: K.P.; S.-w.L.
†These authors contributed equally to this work.
Abstract
Replacing damaged salivary glands with in vitro-generated artificial glands offers a fundamental solution for salivary gland dysfunction. However, this approach remains challenging due to the gland's complex structure and cellular heterogeneity. Since natural organogenesis of salivary glands successfully orchestrates these complex processes, replicating the developmental niche in vitro is considered a promising solution. However, it consists of complex, branched structures formed by multiple factors; thus, recapitulation of these factors in vitro using a single type of biomaterial is difficult to achieve. Therefore, this study aims to design a scaffold capable of spontaneously mimicking salivary gland's developmental niche. Herein, we demonstrate that catechol-incorporated polyacrylonitrile (PAN-C) nanofiber scaffold spontaneously transforms into biomimetic structures by adsorbing embryonic mesenchyme-derived extracellular matrix (ECM) and growth factors. Accumulated adsorption of ECM and growth factors on PAN-C nanofibers promoted the proliferation, morphogenesis, and functional differentiation of embryonic salivary gland (eSG) organoids in vitro. Transcriptome analysis revealed that the PAN-C nanofiber scaffold effectively reduced mechanical stress-induced gene expression while promoting proliferation and differentiation of salivary gland epithelial cells. In eSG organoids cultured on PAN-C nanofiber scaffolds, the proportion of functional acinar cells expressing apically localized aquaporin-5 was substantially higher than those cultured on polycarbonate membranes, a conventional culture material. Therefore, PAN-C nanofiber scaffolds provide an effective and economical method for generating functional eSG organoids in vitro.
논문정보
- Research article
- 2025년 07월 (BRIC 등록일 2025-07-04)
- 국내 연구진
- 의약학 > 치의학
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