한빛사
>
한빛사논문
한빛사논문
- 조회331
Nano Lett., Jun 10 2025, | https://doi.org/10.1021/acs.nanolett.5c01840
Real-Time Intracellular Monitoring of miRNA Dynamics during Induced Pluripotent Stem Cell Neuronal Differentiation via Plasmon-Enhanced Nanobiosensing
Yannan Hou 1, Meizi Chen 1, Letao Yang 1,2, Ki-Bum Lee 1
1Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, United States.
2Shanghai Tongji Hospital, Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration, Ministry of Education, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Corresponding Authors : Letao Yang, Ki-Bum Lee
1Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, United States.
2Shanghai Tongji Hospital, Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration, Ministry of Education, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Corresponding Authors : Letao Yang, Ki-Bum Lee
Abstract
Induced pluripotent stem cells (iPSCs) offer immense potential for treating central nervous system (CNS) disorders and injuries. However, the lack of highly sensitive, selective, and noninvasive biosensors for real-time monitoring of iPSC neuronal differentiation remains a critical barrier. In this work, we introduce a gold nanorod-based metal-enhanced molecular beacon (MEMB) nanobiosensor for the noninvasive, real-time detection of intracellular miRNA-124, a key biomarker for neuronal differentiation in human iPSC-derived neural stem cells. Designed through finite-difference time-domain (FDTD) simulations and experimentally validated for optimized localized surface plasmon resonance (LSPR) properties, MEMB nanobiosensors achieved picomolar-level sensitivity and single-mismatch selectivity toward miRNA-124 detection, along with great biocompatibility demonstrated by live-cell assays. Collectively, the MEMB platform provides a robust analytical tool for in-depth investigations of molecular and genetic regulatory networks during iPSC neuronal differentiation in a nondestructive manner, paving the way toward safer, more efficient, and better-characterized iPSC-derived cell therapies for CNS diseases and injuries.
논문정보
- Research article
- 2025년 06월 (BRIC 등록일 2025-06-13)
- 국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
