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Appl. Phys. Rev., Volume 12, Issue 2 June 2025 | https://doi.org/10.1063/5.0228874
In vivo delivery systems for CRISPR genome editing: Viral and non-viral carriers
Yeeun Lee,1 Bookun Kim,1 Donghyun Lee,1 Seo Young Cheon,1 Seong Gi Lim,1 Younggwang Kim,2,a) and Heebeom Koo1,a)
1Department of Medical Life Sciences and Department of Medical Sciences (Graduate School), College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea
2Department of Pathology and Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea
a)Authors to whom correspondence should be addressed: Younggwang Kim, and Heebeom Koo
1Department of Medical Life Sciences and Department of Medical Sciences (Graduate School), College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea
2Department of Pathology and Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea
a)Authors to whom correspondence should be addressed: Younggwang Kim, and Heebeom Koo
Abstract
For a long time, efficient and safe gene delivery has been a key issue in gene therapy. In particular, after the Nobel Prize in Chemistry for clustered regularly interspaced short palindromic repeat (CRISPR) technology in 2020, the focus on delivery systems for genome editing has grown. In this review, we introduce the recent trends in various CRISPR delivery systems. First, we explain the impact of CRISPR in clinical settings and its history. We then focused on the physics of gene delivery systems, particularly regarding the migration of nanoparticles (NPs) under flow, cellular uptake, and formulation using microfluidics. Subsequently, various CRISPR delivery systems, both viral and non-viral, and their applications in disease therapy were introduced. Viral carriers include lentiviruses, adeno-associated viruses, and viral capsids. Exosomes, silica NPs, polymeric NPs, and lipid NPs are representative non-viral gene delivery carriers. We mainly focused on studies demonstrating promising results in animal models, not stopped at cell test considering their future potential for human application.
논문정보
- Review Paper
- 2025년 06월 (BRIC 등록일 2025-06-10)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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