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Small Methods, Jun 04 2025, | https://doi.org/10.1002/smtd.202500729
The 96-Puddle Paper Plate as a Versatile Lab-on-Paper Platform
Taeha Lee 1,2, Yeongjun Seo 1, Dain Kang 1, Seung Hyeon Oh 1,2, Sang Won Lee 3,4, Insu Park 5, Gyudo Lee 1,2
1Department of Biotechnology and Bioinformatics, Korea University, Sejong, 30019, South Korea.
2Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology, Korea University, Sejong, 30019, South Korea.
3Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, United States.
4Smart City Intelligent Research Center (SCIRC), Information Technology Research Center (ITRC), Korea University, Sejong, 30019, South Korea.
5Department of Biomedical Engineering, Yonsei University, Wonju, 26493, South Korea.
Corresponding Authors: Insu Park, Gyudo Lee
1Department of Biotechnology and Bioinformatics, Korea University, Sejong, 30019, South Korea.
2Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology, Korea University, Sejong, 30019, South Korea.
3Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, United States.
4Smart City Intelligent Research Center (SCIRC), Information Technology Research Center (ITRC), Korea University, Sejong, 30019, South Korea.
5Department of Biomedical Engineering, Yonsei University, Wonju, 26493, South Korea.
Corresponding Authors: Insu Park, Gyudo Lee
Abstract
Microfluidic paper-based analytical devices (µPADs) have facilitated the detection of a wide range of analytes, spanning human health indicators and environmental contaminants. However, their diverse designs necessitate specialized analytical equipment, which complicates standardization, limits productivity, impedes universal analysis, and hinders the acquisition of reliable data. To address these issues, a 96-Puddle Paper Plate (96-PPP) is presented, a versatile lab-on-paper platform that is fully compatible with microplate readers. The 96-PPP offers significant advantages in high-throughput analysis, enabling both qualitative assessments using visual inspection and smartphone cameras, as well as quantitative measurements of absorbance and fluorescence. The versatility of this platform is demonstrated by its ability to detect a wide range of key analytes, including acetone, magnesium ions (Mg2+), ascorbic acid, and ketone, which is pertinent to clinical diagnostics, metabolic research, and environmental monitoring. Additionally, 96-PPP enables the study of amyloid deposition (variation within 3.5% across 480 puddles) and proteolysis in cellulose networks that resemble extracellular matrix structures, a capability not achievable with conventional 96-well plates. Overall, 96-PPP replicates the functionality of 96-well plates and concurrently addresses the limitations of conventional µPADs, such as low productivity, versatility, and reliability. Furthermore, it provides unprecedented scalability via a stable, cellulose-based sheet.
논문정보
- Research article
- 2025년 06월 (BRIC 등록일 2025-06-05)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 다학제 융합/복합
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