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ACS Nano, May 20 2025, | https://doi.org/10.1021/acsnano.5c02283 Ionizable Lipids Drive Subcellular Localization and Immune Cell Targeting of Barcoded Nanoparticles in Lung Cancer

Nuri Oh ¹, Jae Yoon Kim ²

¹Department of Chemistry and Biology, Korea Science Academy of KAIST, Busan 47162, Republic of Korea.
²Department of Urology, Sanggye Paik Hospital, Inje University College of Medicine, Seoul 01757, Republic of Korea.

Corresponding Author : Nuri Oh

Abstract

To accurately predict the effect of a drug and enhance its potency, it is essential to examine not only the arrival of the carrier and its payload at the target cell but also the final destination of the subcellular organelle because a considerable number of diseases are associated with the malfunctioning of cellular organelles. Here, we present nanoparticle (NP) microscopy via signal amplification of DNA barcodes combined with the multiplexed cyclic immunofluorescence technique for quantifying multiple NP types simultaneously. This technique enhanced the fluorescence signal-to-noise by 15-fold compared to standard fluorescence in situ hybridization, thereby providing a more precise means of analyzing the intra- and interdistribution of three core-shell NPs (G0-P5, 7C1-F5, and C12-D) in vitro and in vivo. The in vitro results demonstrated that in macrophages, nucleic acids condensed with G0-C14 cationic lipids were often located in lysosomes, whereas in tumor cells, nucleic acids were mainly located in mitochondria, regardless of the type of cationic lipid. Together, the in vivo results reveal that nucleic acids condensed with G0-C14 cationic lipids demonstrated the greatest uptake by CD206+ immune cells, whereas nucleic acids condensed with 7C1 and C12-200 cationic lipids exhibited the highest level of uptake by CD206+CD11c+Arg1+ immune cells.

논문정보

형식Research article
게재일2025년 05월 (BRIC 등록일 2025-05-22)
연구진국내 연구진
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