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Angew. Chem.-Int. Edit., May 13 2025, | https://doi.org/10.1002/anie.202502159
Spatiotemporal Super-Resolution Imaging of Lipid Metabolism Dynamics in Physiological/Pathological Conditions
Shixian Cao 1, Qihang Ding 2, Caixia Sun 3, Borbala Codogno 1, Zhiqiang Liu 1, Xiaoqiang Yu 1, Jong Seung Kim 4, Kang-Nan Wang 1
1Shandong University, State Key Laboratory of Crystal Materials, CHINA.
2Korea University, Department of Chemistry, KOREA, REPUBLIC OF.
3Nanyang Technological University, School of Chemistry, Chemical Engineering and Biotechnology, SINGAPORE.
4Korea University, Department of Chemistry, Anamdong, 02841, Seoul, KOREA, REPUBLIC OF.
Corresponding Author : Jong Seung Kim
1Shandong University, State Key Laboratory of Crystal Materials, CHINA.
2Korea University, Department of Chemistry, KOREA, REPUBLIC OF.
3Nanyang Technological University, School of Chemistry, Chemical Engineering and Biotechnology, SINGAPORE.
4Korea University, Department of Chemistry, Anamdong, 02841, Seoul, KOREA, REPUBLIC OF.
Corresponding Author : Jong Seung Kim
Abstract
Dysregulated lipid metabolism plays a critical role in the pathogenesis of numerous diseases. However, due to the lack of effective solutions, the dynamic monitoring and deep understanding of lipid metabolism in the progression of diseases has remained challenging. Herein, a polarity-sensitive, lipid droplets (LDs)-targeted probe was developed via molecular surgery for spatiotemporal super-resolution imaging of lipid metabolism dynamics. Based on the stimulated emission depletion (STED) and fluorescence lifetime imaging microscopy (FLIM) platform, we achieved the super-resolution visualization and dynamic tracking of the nonlinear fusion and expansion mechanisms of LDs in orthotopic pathology models-an unprecedented achievement in the field. We also analyzed lipid distribution in zebrafish models using the STED-FLIM platform to capture lipid metabolism at the organismal level. Crucially, based on the integrated STED-FLIM platform, this probe accurately monitors lipid metabolism in cellular and pathological tissue contexts of atherosclerosis and fatty liver. This breakthrough provides novel insights for diagnosing lipid metabolism-related diseases and advancing therapeutic development.
논문정보
- Research article
- 2025년 05월 (BRIC 등록일 2025-05-14)
- 국내(교신)+국외 연구진
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