황은하 (한국생명공학연구원) | 한빛사논문 > 한빛사

한빛사논문

조회73

황은하Eun-Ha Hwang

한국생명공학연구원

J. Hazard. Mater., May 02 2025, 494 S0304-3894(25)01372-X | https://doi.org/10.1016/j.jhazmat.2025.138457 Bioelectronics based on antibody-conjugated graphene field-effect transistor for response of Staphylococcal enterotoxin B as a biological weapon

Kyung Ho Kim^a,1, Eun-Ha Hwang ^b,1, Ju Hong Jang^c,1, Sung Eun Seo ^a, Jung Joo Hong ^b,d, Jangwook Lee ^c,e, Oh Seok Kwon ^a,f,g

^aSKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 16419, Republic of Korea
^bNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Chungcheongbuk 28116, Republic of Korea
^cBiotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
^dKRIBB School of Bioscience, Korea University of Science & Technology (UST), Daejeon 34141, Republic of Korea
^eDepartment of Biomolecular Science, Korea Research Institute of Bioscience and Biotechnology, School of Bioscience, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea
^fDepartment of Nano Science and Technology, Sungkyunkwan University, Suwon 16419, Republic of Korea
^gDepartment of Nano Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Republic of Korea

¹These authors contributed equally tothiswork.These authors contributed equally to this work.
Corresponding authors: Jung Joo Hong, Jangwook Lee, Oh Seok Kwon

Abstract

Staphylococcal enterotoxin B (SEB) is a potent toxin that is produced by Staphylococcus aureus and is classified as an agent for biological weapons. Various nanotechnology-based biosensors have been developed to respond to SEB-based biological weapons; however, these biosensors exhibit various limitations. A novel chimeric antibody was developed from SEB-specific hybridoma clones that were generated using native-like SEB antigen expressed via a baculovirus system. The chimeric antibody exhibited high binding specificity and subnanomolar affinity and was subsequently conjugated onto a graphene field-effect transistor-based bioelectronic (SEB bioelectronic). This device exhibited high performance with a limit of detection of 1 pg/mL and a detection range of 1 pg/mL to 100 ng/mL, demonstrating superior specific detection performance even in the presence of various interference toxin substances at 10³ times higher concentrations. Moreover, the SEB bioelectronic was evaluated using nonhuman primate infection models, and the detection performance was investigated based on those for standard SEB substances. These results indicate that the SEB bioelectronic can be utilized for noncontact SEB detection in SEB-exposed onsite locations and can be applied for bioelectronic development to respond to other biological weapons through bioprobe exchanges.

논문정보

형식Research article
게재일2025년 05월 (BRIC 등록일 2025-05-07)
연구진국내 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기