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서울대학교

Exp. Mol. Med., May 01 2025, | https://doi.org/10.1038/s12276-025-01453-w Analytical techniques for nucleic acid and protein detection with single-molecule sensitivity

Soochul Shin ¹, Juyoung Kim ¹, Eunho Song ¹, Sun Han ¹ and Sungchul Hohng ^1,*

¹Department of Physics and Astronomy, Institute of Applied Physics, Seoul National University, Seoul, Republic of Korea.

^*Corresponding author: correspondence to Sungchul Hohng

Abstract

Nucleic acids and proteins serve as crucial biomarkers used in the diagnosis, prognosis and therapy monitoring across various diseases. Traditionally, these biomarkers are identified at an ensemble level following the amplification of target or signaling molecules. However, these methods have limitations in addressing contemporary challenges in molecular diagnostics, such as low sensitivity, specificity, throughput and imprecise quantification. To overcome these limitations, various analytical techniques offering single-molecule sensitivity have been developed. Here we aim to provide a concise overview of historically notable and potentially promising analytical techniques to detect nucleic acids and proteins with single-molecule sensitivity. Our focus is on their potential in liquid biopsy, delineating their strengths and weaknesses, providing insights into their ability to revolutionize biomarker analysis and paving the way for more advanced technologies.

논문정보

형식Review Paper
게재일2025년 05월 (BRIC 등록일 2025-05-02)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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