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Chem. Eng. J., Volume 512, 15 May 2025, 162541 | https://doi.org/10.1016/j.cej.2025.162541 Highly wrinkled reduced graphene oxide nanocomposite coating for a multiplexed electrochemical biosensor

Su Yeong Kim ^a,1, Min Kim^a,1, Hyowon Jang ^b, Byeongju Jeon ^c, Hanul Kim ^c, Siyoung Q, Choi ^c, Byungkook Oh ^a, Heemin Kang ^d, Taejoon Kang ^b,g, Hyung-Ryong Kim ^e, Jeong-Chan Lee ^f, Steve Park^a,h

^aDepartment of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea
^bBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea
^cDepartment of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea
^dDepartment of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea
^eDepartment of Pharmacology, College of Dentistry, Jeonbuk National University, Jeonju, 54907, Republic of Korea
^fApplied Science Research Institute, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea
^gSchool of Pharmacy, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea
^hKAIST Institute for NanoCentury, Daejeon, 34141, Republic of Korea

¹These authors contributed equally.
Corresponding authors : Jeong-Chan Lee, Steve Park

Abstract

Nanomaterial-based electronic devices, with dimensions akin to biological molecules and unique chemical attributes, play a pivotal role in various biomedical applications. However, achieving cost-effective and large-scale fabrication of nanomaterial-based thin films while precisely controlling nanostructures poses considerable challenges, hindering their widespread industrial adoption. To address this, we introduce meniscus-guided coating for the fabrication of reduced graphene oxide nanocomposite with high surface area and excellent electroconductive properties in a facile and scalable manner. Using computational fluid dynamics modeling, we systematically analyzed the rheological properties of the solution containing reduced graphene oxide and chitosan, optimizing coating speed conducive to the stable formation of an elongated meniscus. Additionally, we employed in situ high-speed microscopy to observe the solidification kinetics, enabling an understanding of the wrinkle formation mechanism that enhances the overall surface area. Compared to thicker coatings (∼hundreds of nanometers), the optimized nanocomposite exhibited a 62% increase in surface area and a 97% enhancement in electroconductivity. Consequently, a multiplexed biosensor featuring this highly wrinkled thin nanocomposite simultaneously detected three antibodies related to endoplasmic reticulum stress on the same chip without any cross-reactivity: anti-PDIA6, anti-PERK, and anti-GRP78, with limit of detection of 65.94 pg mL−1, 102.58 pg mL−1, 53.90 pg mL−1, respectively. This nanomaterial coating technology holds promise in overcoming barriers that hinder precise control of nanostructures within thin films, facilitating the transition to mass-production of the multiplexed sensors in biomedical fields, such as point-of-care diagnostics and healthcare monitoring.

논문정보

형식Research article
게재일2025년 05월 (BRIC 등록일 2025-04-29)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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