한빛사
>
한빛사논문
한빛사논문
- 조회103
Adv. Funct. Mater., 24 April 2025 | https://doi.org/10.1002/adfm.202417935
Hypoxia‐Sensitive Smart Hydrogel Biosensor for Distinct Mechanical and Electrical Signals with Muscle Ischemia Regeneration
Sunu Hangma Subba1, Songling Jiang2, Eun-Jung Jin3, Sung Young Park1,4
1Department of IT and Energy Convergence (BK21 FOUR), Korea National University of Transportation, Chungju, 27469 Republic of Korea
2Integrated Omics Institute, Wonkwang University, Iksan, Jeonbuk, 54538 South Korea
3Department of Biomedical Materials Science, Graduate School of JABA, Wonkwang University, Iksan, Jeonbuk, 54538 Republic of Korea
4Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju, 27469 Republic of Korea
Corresponding Authors: Eun-Jung Jin, Sung Young Park
1Department of IT and Energy Convergence (BK21 FOUR), Korea National University of Transportation, Chungju, 27469 Republic of Korea
2Integrated Omics Institute, Wonkwang University, Iksan, Jeonbuk, 54538 South Korea
3Department of Biomedical Materials Science, Graduate School of JABA, Wonkwang University, Iksan, Jeonbuk, 54538 Republic of Korea
4Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju, 27469 Republic of Korea
Corresponding Authors: Eun-Jung Jin, Sung Young Park
Abstract
A hypoxia-specific diselenide-crosslinked polymer dot (PD) nanoparticles-based pyrogallol-modified hydrogel (HS-PD hydrogel) is developed for the facile monitoring of muscle ischemia recovery through multifaceted mechanical, electrical, and optical modulation. The ischemia environment-sensitive conductive hydrogel exploits the specific cleavage of diselenide bonds induced by overexpressed reactive oxygen species (ROS), regulating fluorescence “on/off” activation, and subsequently modifies the microstructural morphology of the matrix. The HS-PD hydrogel utilizes the oxidizing properties of pyrogallol to modulate its electroconductivity (ΔR decreased by ≈78.1%) and mechanophysical properties under normoxic conditions while enabling naked-eye detection via visible color changes. Moreover, in vitro mechanophysical and electrical changes are evidenced by an increase in stretchability and compression modulus, alongside reduced electrical resistivity under normoxic conditions, as confirmed using C2C12 and 3T3-L1 cell models. Additionally, in vitro gene expression analysis shows significant downregulation of SOD2, Hif-1α, and MuRF-1 genes associated with muscle degradation, indicating enhanced ROS scavenging and potential oxygen normalization in ischemic regions. In vivo studies using a murine model of femoral artery ligation show a reduced inflammatory response, muscle fiber hypertrophy, and increased regenerative capacity in ischemic tissues. These findings highlight the potential of hydrogels in muscle regeneration and therapeutic applications.
논문정보
- Research article
- 2025년 04월 (BRIC 등록일 2025-04-26)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
관련분야 논문보기
