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조회112

조성연Seongyeon Cho

서울대학교

Biosens. Bioelectron., Apr 01 2025, 281 S0956-5663(25)00307-0 | https://doi.org/10.1016/j.bios.2025.117433 Nondestructive methodology with discerning fruit freshness biomarker compounds using olfactory receptor nanodisc-loaded graphene transistors

Sung Eun Seo ^a,1, Seongyeon Cho ^b,1, Kyung Ho Kim ^a,1, Seongbin Lee ^c, Jiwon Lee ^c, Yeon Kyung Cha ^d, Yong-Sang Ryu ^e,f,g, Tai Hyun Park ^b,h, Hyun Seok Song ^d,i, Oh Seok Kwon ^a,j,k

^aSungkyunkwan SKKU Advanced Institute of Nano Technology (SAINT), Sungkyunkwan University, Suwon, 16419, Republic of Korea
^bSchool of Chemical and Biological Engineering, Institute of Chemical Process, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea
^cCenter for Climate and Carbon Cycle Research, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea
^dSensor System Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
^eDepartment of Micro/Nano Systems, Korea University, Seoul, 02841, Republic of Korea
^fSchool of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea
^gBK21 Four Institute of Precision Public Health, Korea University, Seoul, Republic of Korea
^hDepartment of Nutritional Science and Food Management, Ewha Womans University, Seoul, 03760, Republic of Korea
ⁱKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea
^jDepartment of Nano Science and Technology, Sungkyunkwan University, Suwon, 16419, Republic of Korea
^kDepartment of Nano Engineering, Sungkyunkwan University, Suwon, Republic of Korea

¹These authors contributed equally to this work.
Corresponding authors: Tai Hyun Park, Hyun Seok Song, Oh Seok Kwon

Abstract

Noninvasive and facile methodologies for the assessment of fruit freshness are essential for the determination of fruit ripeness. Fruits emit characteristic aroma molecules during maturation, and the selective and sensitive detection of these scents is highly challenging. Herein, a novel nondestructive methodology for discerning fruit freshness biomarker compounds based on a target-specific olfactory receptor-loaded transistor for detecting aroma molecules at ppb levels is proposed. This fruit freshness discrimination platform can detect the representative aromas caused by compounds including iso-amyl acetate, amyl-butyrate, geraniol, and β-citronellol that are emitted by each fruit species, such as bananas, grapes, and apricots, and has a lower limit of detection below femtomolar concentrations. Measurement of the selective binding affinity between the olfactory receptor and aroma molecules was conducted using biosimulation, and the experimental results were compared with the sensor response. The fabricated sensor exhibited superior performance in determining the ripeness of fruits with discerning fruit freshness biomarker compounds and presented promising potential for application in agriculture and other industry fields as an assessment platform for detecting various aromatic molecules. Our findings highlight the broad utilization of newly developed sensor platforms as standards for distinguishing freshness.

논문정보

형식Research article
게재일2025년 04월 (BRIC 등록일 2025-04-14)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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