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Northwestern University

Neuron, April 10, 2025 | https://doi.org/10.1016/j.neuron.2025.03.017 An integrated microfluidic and fluorescence platform for probing in vivo neuropharmacology

Sean C. Piantadosi,^1,2,17 Min-Kyu Lee,^3,4,5,6,17 Mingzheng Wu,^3,7,17 Huong Huynh,^3,8 Raudel Avila,^10,12 Catalina A. Zamorano,^1,2,9 Carina Pizzano,^1,2 Yixin Wu,^3,11 Rachael Xavier,⁴ Maria Stanslaski,⁴ Jiheon Kang,³ Sarah Thai,^1,2 Youngdo Kim,^3,11 Jinglan Zhang,⁷ Yonggang Huang,^3,11,12,13 Yevgenia Kozorovitskiy,^7,14 Cameron H. Good,^3,4 Anthony R. Banks,^3,4 John A. Rogers,^{3,11,12,15,18,*} and Michael R. Bruchas ^{1,2,9,16,18,19,*}

¹Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA
²Center of Excellence in Neurobiology of Addiction, Pain, and Emotion (NAPE), University of Washington, Seattle, WA, USA
³Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA
⁴Neurolux Inc., Northfield, IL 60093, USA
⁵Shirley Ryan AbilityLab, Chicago, IL 60611, USA
⁶Department of Physical Medicine and Rehabilitation, Northwestern University, Chicago, IL 60611, USA
⁷Department of Neurobiology, Northwestern University, Evanston, IL, USA
⁸Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA
⁹Department of Pharmacology, University of Washington, Seattle, WA, USA
¹⁰Department of Mechanical Engineering, Rice University, Houston, TX, USA
¹¹Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA
¹²Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA
¹³Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208, USA
¹⁴Chemistry of Life Processes Institutes, Northwestern University, Evanston, IL, USA
¹⁵Department of Neurological Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
¹⁶Department of Bioengineering, University of Washington, Seattle, WA, USA
¹⁷These authors contributed equally
¹⁸Senior author
¹⁹Lead contact

^*Corresponding authors: correspondence to John A. Rogers or Michael R. Bruchas

Abstract

Neurotechnologies and genetic tools for dissecting neural circuit functions have advanced rapidly over the past decade although the development of complementary pharmacological methodologies has comparatively lagged. Understanding the precise pharmacological mechanisms of neuroactive compounds is critical for advancing basic neurobiology and neuropharmacology, as well as for developing more effective treatments for neurological and neuropsychiatric disorders. However, integrating modern tools for assessing neural activity in large-scale neural networks with spatially localized drug delivery remains a major challenge. Here, we present a dual microfluidic-photometry platform that enables simultaneous intracranial drug delivery with neural dynamics recording in the rodent brain. The integrated platform combines a wireless, battery-free, miniaturized fluidic microsystem with optical probes, allowing for spatially and temporally restricted drug delivery while sensing activity-dependent fluorescence using genetically encoded calcium indicators (GECIs), neurotransmitter sensors, and neuropeptide sensors. We demonstrate the performance of this platform for investigating neuropharmacological mechanisms in vivo in behaving mice.

논문정보

형식Research article
게재일2025년 04월 (BRIC 등록일 2025-04-14)
연구진국외 연구진
분야 바이오·의료융합 > 뇌인지과학

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