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한빛사논문
- 조회77
Yevheniia KharkivskaYevheniia Kharkivska
한국과학기술연구원, UST
Phytomedicine, Apr 08 2025, 141 S0944-7113(25)00323-X | https://doi.org/10.1016/j.phymed.2025.156683
Dual effects of Korean red ginseng extract and its fractions on influenza A virus infectivity in lung-derived cell lines
Yevheniia Kharkivska a,b,1, Dong Hoon Kim c,1, Olha Shkel a,b, Sun-Hak Lee d, Young Taek Jeong c, Yun Kyung Kim a,b, Chang Sun Song d, Jun-Seok Lee c
aDivision of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology (UST), Seoul 02792, South Korea
bBrain Disorders Research Center, Brain Science Research Division, Korea Institute of Science and Technology (KIST), Seoul 02792, South Korea
cDepartment of Pharmacology, College of Medicine, Korea University, 73 Goryeodae-ro, Seongbuk-gu, Seoul 02841, South Korea
dCollege of Veterinary Medicine, Konkuk University, Seoul, South Korea
1These authors contributed equally to this work.
Correspondence authors: Yun Kyung Kim, Chang Sun Song, Jun-Seok Lee
aDivision of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology (UST), Seoul 02792, South Korea
bBrain Disorders Research Center, Brain Science Research Division, Korea Institute of Science and Technology (KIST), Seoul 02792, South Korea
cDepartment of Pharmacology, College of Medicine, Korea University, 73 Goryeodae-ro, Seongbuk-gu, Seoul 02841, South Korea
dCollege of Veterinary Medicine, Konkuk University, Seoul, South Korea
1These authors contributed equally to this work.
Correspondence authors: Yun Kyung Kim, Chang Sun Song, Jun-Seok Lee
Abstract
Background: Influenza infections are significantly affected by the genetics of the viruses and the cells they infect. Our previous studies showed that various influenza A subtypes uniquely infect different cell lines, offering insights into viral infection mechanisms. Meanwhile, Korean red ginseng extract (RGE) is known for its anti-influenza properties, attributed to its rich composition of saponin and non-saponin components.
Purpose: This study evaluates the antiviral effects of RGE and its non-saponin (GNSF) and saponin (GSF) fractions against H1N1 and H9N2 influenza A subtypes in diverse cell lines.
Study design: Using various cell types and specialized assays, we explored the effect of pretreatment and continuous treatment with RGE and its fractions on viral infectivity and subsequent cellular responses.
Methods: We treated several cell lines with varying concentrations of RGE, GNSF, and GSF and measured the cytotoxic effect, viral infectivity, oxidative stress levels, immune responses, autophagy activity, and changes in cellular structure.
Results: Our findings demonstrate that RGE and its fractions significantly reduced H9N2 infection levels across multiple cell lines under pretreatment and continuous treatment conditions. However, continuous treatment elicited variable responses to H1N1, with increased infection levels in certain cell lines. Additionally, it elevated the production of reactive oxygen species and altered inflammatory responses, especially in A549 and NCI-H292 cells. GSF also modulated autophagy activity and MUC1 expression in response to H1N1.
Conclusion: These findings highlight the potential of ginseng components as targeted influenza treatment, with cell line-specific responses that could guide treatment approaches.
Purpose: This study evaluates the antiviral effects of RGE and its non-saponin (GNSF) and saponin (GSF) fractions against H1N1 and H9N2 influenza A subtypes in diverse cell lines.
Study design: Using various cell types and specialized assays, we explored the effect of pretreatment and continuous treatment with RGE and its fractions on viral infectivity and subsequent cellular responses.
Methods: We treated several cell lines with varying concentrations of RGE, GNSF, and GSF and measured the cytotoxic effect, viral infectivity, oxidative stress levels, immune responses, autophagy activity, and changes in cellular structure.
Results: Our findings demonstrate that RGE and its fractions significantly reduced H9N2 infection levels across multiple cell lines under pretreatment and continuous treatment conditions. However, continuous treatment elicited variable responses to H1N1, with increased infection levels in certain cell lines. Additionally, it elevated the production of reactive oxygen species and altered inflammatory responses, especially in A549 and NCI-H292 cells. GSF also modulated autophagy activity and MUC1 expression in response to H1N1.
Conclusion: These findings highlight the potential of ginseng components as targeted influenza treatment, with cell line-specific responses that could guide treatment approaches.
논문정보
- Research article
- 2025년 04월 (BRIC 등록일 2025-04-10)
- 국내 연구진
- 의약학 > 약리의학
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