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ACS Nano, Mar 27 2025, | https://doi.org/10.1021/acsnano.4c18297
Vertically Aligned Nanowires for Longitudinal Intracellular Sampling
David Eun Reynolds 1, Yoon Ho Roh 2,3, Uday Chintapula 2, Emily Huynh 1, Phoebe Vallapureddy 1, Hong Huy Tran 4,5, Daeyeon Lee 4, Mark G Allen 6, Xiaowei Xu 2, Jina Ko 1,2
1Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
2Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
3Department of Energy and Chemical Engineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea.
4Department of Chemical and Biomolecular Engineering, School of Engineering & Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
5Center for Innovation & Precision Dentistry, School of Dental Medicine, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
6Department of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
D.E.R. and Y.H.R. contributed equally to this work.
Corresponding Author: Jina Ko
1Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
2Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
3Department of Energy and Chemical Engineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea.
4Department of Chemical and Biomolecular Engineering, School of Engineering & Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
5Center for Innovation & Precision Dentistry, School of Dental Medicine, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
6Department of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
D.E.R. and Y.H.R. contributed equally to this work.
Corresponding Author: Jina Ko
Abstract
Cells are diverse systems with unique molecular profiles that support vital functions, such as energy production and nutrient absorption. Advances in omics have provided valuable insights into these cellular processes, but many of these tools rely on cell lysis, limiting the ability to track dynamic changes over time. To overcome this, methods for longitudinal profiling of living cells have emerged; however, challenges such as low throughput and genetic manipulation still need to be addressed. Nanomaterials, particularly nanowires, offer a promising solution due to their size, high aspect ratios, low cost, simplicity, and potential for high-throughput manufacturing. Here, we present a nanowire-based platform for longitudinal mRNA profiling in living cells using vertically aligned nickel nanowire arrays for efficient mRNA extraction with minimal cellular disruption. We demonstrate its ability to track enhanced green fluorescent protein expression and transcriptomic changes from drug responses in the same cells over time, showcasing the platform's potential for dynamic cellular analysis.
논문정보
- Research article
- 2025년 03월 (BRIC 등록일 2025-05-09)
- 국외 연구진
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