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한빛사논문
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Biosens. Bioelectron., Available online 8 March 2025 | https://doi.org/10.1016/j.bios.2025.117357
Amplifying visible signals in lateral flow assays using integrated nanoelectrokinetics
Jeong Soo Park a,b,1, Ji Hye Hong a,c,1, Seungmin Lee a,c, Seok Chung b, Dae Sung Yoon c,d, Seungmin Kwak e, Dohwan Lee f, Jeong Hoon Lee a,g
aKU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk, Seoul, 02841, Republic of Korea
bSchool of Mechanical Engineering, Korea University, 145 Anam-ro, Seongbuk, Seoul, 02841, Republic of Korea
cSchool of Biomedical Engineering, Korea University, 145 Anam-ro, Seongbuk, Seoul, 02841, Republic of Korea
dInterdisciplinary Program in Precision Public Health, Korea University, Seoul, 02841, Republic of Korea
eMicro-Nano Fabrication Center, Korea Institute of Science and Technology, 5 Hwarang-ro 14-gil, Seongbuk, Seoul, 02792, Republic of Korea
fDepartment of Electrical Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon, Seoul, 01897, Republic of Korea
gDepartment of Integrative Energy Engineering, Korea University, 145 Anam-ro, Seongbuk, Seoul, 02841, Republic of Korea
1These authors contributed equally.
Corresponding authors: Dohwan Lee, Jeong Hoon Lee
aKU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk, Seoul, 02841, Republic of Korea
bSchool of Mechanical Engineering, Korea University, 145 Anam-ro, Seongbuk, Seoul, 02841, Republic of Korea
cSchool of Biomedical Engineering, Korea University, 145 Anam-ro, Seongbuk, Seoul, 02841, Republic of Korea
dInterdisciplinary Program in Precision Public Health, Korea University, Seoul, 02841, Republic of Korea
eMicro-Nano Fabrication Center, Korea Institute of Science and Technology, 5 Hwarang-ro 14-gil, Seongbuk, Seoul, 02792, Republic of Korea
fDepartment of Electrical Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon, Seoul, 01897, Republic of Korea
gDepartment of Integrative Energy Engineering, Korea University, 145 Anam-ro, Seongbuk, Seoul, 02841, Republic of Korea
1These authors contributed equally.
Corresponding authors: Dohwan Lee, Jeong Hoon Lee
Abstract
In this study, we developed a tangential nanoelectrokinetic force-enhanced lateral flow assay by integrating a Nafion-cellulose nanofibrils hybrid gel to improve conventional lateral flow assay performance without external instrumentation. Our assay was designed by embedding the Nafion-cellulose nanofibrils gel beneath the test line, generating a tangential nanoelectrokinetic force that enhances the migration and accumulation of antigen-AuNP probe complexes within the visible depth of the nitrocellulose surface. The proposed assay exhibited a 32.5-fold improvement in the limit of detection for COVID-19 nucleocapsid protein, achieving a 0.24 ng/mL. Clinical trials demonstrated 100% sensitivity and 100% specificity, far surpassing the performance of conventional lateral flow assays, which typically achieve 66.7 % sensitivity and 100 % specificity. Our assay also demonstrated high selectivity, with no cross-reactivity against other respiratory pathogens such as respiratory syncytial virus A, respiratory syncytial virus B, OC43 (COVID), influenza A, and influenza B, making it a robust and cost-effective solution for point-of-care diagnostics, particularly in resource-limited settings.
논문정보
- Research article
- 2025년 03월 (BRIC 등록일 2025-03-31)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
관련 웨비나
![나노전기운동력을 활용한 고감도 신속 면역진단 기술 [Biosens. Bioelectron.]](/upload/board/files/onseminar/thumb/thumb_0000945.png)
학술웨비나
나노전기운동력을 활용한 고감도 신속 면역진단 기술 [Biosens. Bioelectron.] 나피온-셀룰로오스 나노섬유 하이브리드 젤을 테스트 라인 아래에 삽입해 이온 공핍력(Ion Depletion Force)을 유도, 항원-금나노입자 복합체가 나이트로셀룰로오스 멤브레인 표면(육안으로 측정 가능한 깊이인 ~10 µm내)에서 항원-항체 결합 반응을 효과적으로 일어날 수 있도록 설계함. 이를 통해 기존 LFA 대비COVID-19 뉴클레오캡시드 단백질 검출 한계를 32.5배 향상(0.24 ng/mL)시켰으며 임상시험 결과 100% 민감도와 100% 특이도를 기록해 기존 LFA(66.7% 민감도, 100% 특이도) 대비 뛰어난 성능을 입증함. 또한 본 기술은 호흡기세포융합바이러스A·B, 코로나OC43, 인플루엔자A·B 등 다른 호흡기 병원체와의 교차반응이 없으며 별도의 장비 없이 간단한 형태로 적용 가능해 저비용·고효율의 현장진단 솔루션으로 활용될 수 있음. 나아가 TNF-LFA 기술은COVID-19를 넘어 다양한 LFA 키트 및 Colorimetric 바이오센서에도 적용 가능한 플랫폼 기술로 확장될 수 있어 감염병 진단뿐만 아니라 생물학적 분석 및 의료 진단 분야에서 다양한 응용이 기대됨.- 홍지혜(고려대학교)
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