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J. Nanobiotechnol., Mar 23 2025, 23 (1) | https://doi.org/10.1186/s12951-025-03287-0
Nanocomposite-based PCR reactors to enhance thermal rate and fluorescence intensity in hand-held qPCR device
Jiyeon Han1,2,4†, Tiara A M1,2,3†, Seongryeong Kim1,4†, Gabriela Morales Florez4, Kiran Shrestha2, Dang Du Nguyen1,4, Inki Kim1,2,4*, Jinkee Lee2,5* and Gyoujin Cho1,2,3,4*
1Department of Biophysics, Sungkyunkwan University, Suwon 16419, South Korea.
2Institute of Quantum Biophysics, Sungkyunkwan University, Suwon 16419, South Korea.
3Research Engineering Center for R2R Printed Flexible Computer, Sungkyunkwan University, Suwon 16419, South Korea.
4Department of Intelligent Precision Healthcare Convergence, Sungkyunkwan University, Suwon 16419, South Korea.
5School of Mechanical Engineering, Sungkyunkwan University, Suwon 16419, South Korea
†Jiyeon Han, A. M. Tiara, and Seongryeong Kim are share the frst authorship equally.
*Correspondence: Inki Kim, Jinkee Lee, Gyoujin Cho
1Department of Biophysics, Sungkyunkwan University, Suwon 16419, South Korea.
2Institute of Quantum Biophysics, Sungkyunkwan University, Suwon 16419, South Korea.
3Research Engineering Center for R2R Printed Flexible Computer, Sungkyunkwan University, Suwon 16419, South Korea.
4Department of Intelligent Precision Healthcare Convergence, Sungkyunkwan University, Suwon 16419, South Korea.
5School of Mechanical Engineering, Sungkyunkwan University, Suwon 16419, South Korea
†Jiyeon Han, A. M. Tiara, and Seongryeong Kim are share the frst authorship equally.
*Correspondence: Inki Kim, Jinkee Lee, Gyoujin Cho
Abstract
A photonic quantitative polymerase chain reaction (qPCR) has usually implemented a polydimethylsiloxane (PDMS) based disposable inexpensive PCR reactor, worked as the photothermal cycler, to show potential as a point-of-care test (PoCT) for detection nucleic acids. However, the PoCT type photonic qPCR has to overcome the prolonged time for the fabrication of PDMS-based PCR reactors and enable a rapid thermal cycler to shorten diagnosis time with a strong fluorescence intensity. Here, we developed a room-temperature curable titanium dioxide (TiO2) nanoparticle dispersed PDMS (TiO2-PDMS) nanocomposite to reduce the fabrication time of the PCR reactor which enhanced the speed of photothermal cycles and fluorescence signal intensity of photonic qPCR. The TiO2-PDMS nanocomposite was formulated for rapid cross-linking at the room-temperature by introducing an optimized amount of Pt catalyst, resulting in the fabrication of a nanocomposite-based PCR reactor within 8 min at room-temperature. The nanocomposite-based PCR reactor enhanced the heating rate to 18.33 Cº/s and cooling rate to -3.11Cº/s because of the phonon scattering effect of TiO2 in the reactor and successfully amplified λ-DNA (amplicon size of 100 bp) within 10 min. Finally, we improved the qPCR efficiency by 2 cycle threshold (Ct) value compared with pristine PDMS reactor and quantified up to 10 copies/µL nucleic acids by fluorescence intensity enhancement resulting from light reflections property of TiO2. By using TiO2-PDMS nanocomposite-based PCR reactors, the fast and efficient nucleic acid assay was enabled without loss of sensitivity, and it can be practically used in the field of PoCT.
논문정보
- Research article
- 2025년 03월 (BRIC 등록일 2025-03-25)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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