김상태 (분당서울대학교병원) | 한빛사논문 > 한빛사

한빛사논문

조회160

김상태SANG TAE KIM

분당서울대학교병원

Biosens. Bioelectron., Available online 7 March 2025 | https://doi.org/10.1016/j.bios.2025.117319 Reagentless aptamer based on the ultrasensitive and fast response electrochemical capacitive biosensor for EGFR detection in non-small cell lung cancer

Enkhzaya Ganbold ^a,b,c,1, Nam Young Kim ^a,b,c,h,1, Yu Mi Kim ^a,e,1, Parshant Kumar Sharma ^a,b, Do Nam Lee ^a,d, Byeolnim Oh ^a,b, Hyun Soo Kim ^a,b, Junghan Song ^a,f, Byungheon Lee ^a,g, Eun-Seong Kim ^a,h,*, Young Kee Shin ^a,h,**, Jeong Su Park ^a,f,***, Sang Tae Kim ^a,e,****

^aRFIC Bio Centre, Kwangwoon University, 20 Kwangwoon-ro, Nowon-Gu, Seoul 01897, Republic of Korea
^bDepartment of Electronics Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon-Gu, Seoul 01897, Republic of Korea
^cNDAC Centre, Kwangwoon University, 20 Kwangwoon-ro, Nowon-Gu, Seoul 01897, Republic of Korea
^dIngenium College of Liberal Arts (Chemistry), Kwangwoon University, 20 Kwangwoon-ro, Nowon-Gu, Seoul 01897, Republic of Korea
^eNeuroscience Research Institute, JnPharma Inc. Healthcare Innovation Park, Seoul National University College of Medicine, Goomi-ro, Bundanggu, Seongnam City, Gyeonggi-do 13605, Republic of Korea
^fDepartment of Laboratory Medicine, Seoul National University Bundang Hospital, Goomi-ro, Bundanggu, Seongnam City, Gyeonggi-do 13605, Republic of Korea
^gDepartment of Biochemistry, School of Medicine, Kyungpook National University 680 Guckchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea
^hDepartment of Molecular Medicine and Biopharmaceutical Sciences, Seoul National University, Seoul 08826, Republic of Korea

^*Corresponding author.
^**Corresponding author.
^***Corresponding author.
^**** Corresponding author.
¹These authors contributed equally to this work.

Abstract

Non-small cell lung cancer (NSCLC) is still the leading cause of lung cancer-related deaths globally, affecting both men and women. Mutations in the epidermal growth factor receptor (EGFR) are most common among patients with NSCLC, especially Asian patients. Here, we introduce an electrochemical capacitive biosensor for the early detection of NSCLC through specific identification of EGFR. A novel and reagentless EGFR aptamer was designed using the systematic evolution of ligands by exponential enrichment (SELEX) process and immobilized on a chromium (Cr)/gold (Au) electrode, with capacitance signals used for detection. The biosensor employs an interdigitated capacitor electrode (IDCE) functionalized with 3-mercaptopropionic acid (MPA), enhancing EGFR aptamer immobilization, while 6-mercapto-1-hexanol (MCH) was used for effective blocking to ensure robust and high-affinity binding to target analytes. The IDCE capacitive biosensor achieved real-time rapid detection within 3 s and demonstrated a detection limit of 0.005 ng/mL for the EGFR peptide, with a dynamic range of 10−11–10−7 ng/mL. Furthermore, the specific EGFR aptamer-immobilized IDCE biosensor was found to be regenerable and reusable up to five times using deionized water. This biosensor offers a rapid, label-free, and highly selective approach for early-stage EGFR detection in NSCLC. Its portability and scalability make it a promising tool for point-of-care diagnostic applications in biomedicine, potentially advancing the field of cancer diagnostics.

논문정보

형식Research article
게재일2025년 03월 (BRIC 등록일 2025-03-20)
연구진국내 연구진
분야 바이오·의료융합 > 의료기기

댓글 작성 로그인

CV 열람하기

연락처 보기