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한빛사논문

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홍용근Yonggeun Hong

인제대학교

CV File 141 kb

Biosens. Bioelectron., Mar 12 2025, 278 S0956-5663(25)00249-0 | https://doi.org/10.1016/j.bios.2025.117375 Nanozyme film with dicopper-coordinated amino-ligands: A dual enzyme-mimic for real-time in situ dopamine sensing in human neuroblastoma cells

Rajesh Madhuvilakku ^a,b,c, Hong Jin Choi ^d, Ok Chan Jeong ^d,e, Yonggeun Hong ^a,b,c,d,f

^aDepartment of Physical Therapy, College of Biomedical Science & Health, Inje University, Gimhae, 50834, Republic of Korea
^bBiohealth Products Research Center (BPRC), Inje University, Gimhae, 50834, Republic of Korea
^cResearch Center for Aged-life Redesign (RCAR), Inje University, Gimhae, 50834, Republic of Korea
^dDepartment of Digital Anti-aging Healthcare, Graduate School of Inje University, Inje University, Gimhae, 50834, Republic of Korea
^eDepartment of Biomedical Engineering, College of Biomedical Science & Health, Gimhae, 50834, Republic of Korea
^fDepartment of Rehabilitation Science, Graduate School of Inje University, Gimhae, 50834, Republic of Korea

Corresponding author : Yonggeun Hong

Abstract

Dopamine (DA) is a vital neurotransmitter, and its real-time detection is critical for understanding neurological functions and diagnosing disorders. Integrated sensing platforms with multiple signal outputs enable real-time monitoring of neurotransmitter release, but their development is often limited by material adaptability, signal reliability, and precise analyte detection in complex systems. Nanozyme-based sensors have emerged as a promising alternative, though developing highly active and selective nanozymes remains a key focus. Herein, a dual-mode portable biosensing device using a nanostructured Cu(II)-poly-L-histidine film via simple electropolymerization onto a screen-printed graphitic electrode (PolyCuHis/SPGE) was developed. This film exhibits remarkable laccase-like, catechol oxidase, and electrocatalytic properties, functioning as an electrochemical/colorimetric probe for DA detection. The sensing mechanism relies on electrostatic and π-π interactions between DA and overoxidized imidazole groups, coupled with efficient charge transfer at active sites, ensuring selective and sensitive DA detection in real-world samples. The PolyCuHis/SPGE sensor demonstrated exceptional performance, with linear responses for electrochemical and colorimetric DA detection in the ranges of 10 nM-100 μM and 1-250 μg/mL, and limits of detection (LOD) of 2.8 nM and 0.204 μg/mL, respectively. The system also exhibited robust repeatability, high stability, and excellent selectivity. Additionally, the device was adapted for visual DA quantification using a smartphone, enhancing its practicality for point-of-care testing (POCT) applications. The sensor's effectiveness was validated by accurately quantifying DA in complex samples and successfully tracking DA release from human neuroblastoma SHSY-5Y cells under pharmacological stimulation. This approach provides a powerful platform for early diagnosis of neurological disorders and advanced POCT applications in neuroscience and clinical diagnostics.

논문정보

형식Research article
게재일2025년 03월 (BRIC 등록일 2025-03-19)
연구진국내 연구진
분야 바이오·의료융합 > 뇌인지과학

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