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고려대학교

Adv. Healthc. Mater., Mar 16 2025, | https://doi.org/10.1002/adhm.202403842 Fibrosis Drug Efficacy Assessment Based on Microfluidic Mechanical Property Evaluation of Spheroid Models

Bolam Kim¹, Jeong Yeon Kim ¹, Hye Won Kim ¹, In Yeong Cho ¹, Ki Wan Bong ¹

¹Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Republic of Korea.

B.K. and J.Y.K. contributed equally to this work.
Corresponding Author : Ki Wan Bong

Abstract

Fibrotic diseases, such as pulmonary fibrosis, pose significant challenges in both research and treatment. To address the limitations of existing systems, a novel collision-based spheroid mechanical property assessment system is developed. The system utilizes inertial fluid dynamics to induce controlled collisions through uniformly sized spheroids, allowing strain to be measured via high-speed cameras. In this study, the system is first validated using HEK293T spheroids to optimize flow velocity, followed by an analysis of deformability differences between two cell types related to pulmonary fibrosis (Calu-1 and MRC-5). A co-culture spheroid model comprising two types of lung cells, endothelial and fibroblast cells, in different rations is further developed, and significant differences in deformability depending on the cell composition is observed. Finally, spheroids are treated with TGF-β1(Transforming Growth Factor-β1), a factor known to activate fibroblast cells and induce excessive extra cellular matrix (ECM) accumulation, and Nintedanib, an anti-fibrotic drug, to assess changes in mechanical properties. These results effectively reflect the mechanical properties driven by cell-cell and cell-ECM interactions and highlight the correlation between spheroid mechanics and the progression of fibrotic disease. This system not only contributes to a deeper understanding of fibrosis progression but also serves as a powerful platform for accelerating the development of anti-fibrotic therapies.

논문정보

형식Research article
게재일2025년 03월 (BRIC 등록일 2025-03-18)
연구진국내 연구진
분야 바이오·의료융합 > 다학제 융합/복합

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