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조회107

한양대학교

Trac-Trends Anal. Chem., 8 March 2025, 118228 | https://doi.org/10.1016/j.trac.2025.11822 Illuminating extracellular vesicles with advanced fluorescence biosensing technologies

Yeonju Lee ^a,1, Kyung-Min Kim ^a,1, Joonseok Lee ^b,c,d, Young-Pil Kim ^a,c,d,e,f

^aDepartment of Life Science, Hanyang University, Seoul 04763, Republic of Korea
^bDepartment of Chemistry, Hanyang University, Seoul 04763, Republic of Korea
^cResearch Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Republic of Korea
^dResearch Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea
^eInstitute of Nano Science and Technology, Hanyang University, Seoul 04763, Republic of Korea
^fHanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea

Corresponding authors: Joonseok Lee, Young-Pil Kim

Abstract

Extracellular vesicles (EVs) are the enigmatic messengers of intercellular communications and vital biological processes, including immune regulation, tissue regeneration, and disease progression. Despite their immense values as clinical diagnosis, the analysis of EVs poses significant challenges due to their small size and heterogeneity. This review focuses on the rapid evolution of fluorescence (FL) biosensing technologies. The convergence of FL biosensing technologies with advancements in biotechnology and nanotechnology has elaborated EV analysis, providing unprecedented sensitivity, specificity, and multiplexing capabilities. Here we overview three key areas of FL analyses of EVs: FL signal amplification technologies, Förster resonance energy transfer-based technologies, and innovative FL technologies. These cutting-edge methods, utilizing fluorescent dyes, enable precise visualization and simultaneous analysis of multiple EVs, offering insights into their diverse subpopulations and potential implications for biological processes, paving the way for novel diagnostic and therapeutic strategies.

논문정보

형식Review Paper
게재일2025년 03월 (BRIC 등록일 2025-03-11)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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