한빛사
>
한빛사논문
한빛사논문
- 조회159
Nat. Commun., 08 March 2025 | https://doi.org/10.1038/s41467-025-57323-6
Microbial metabolites control self-renewal and precancerous progression of human cervical stem cells
Jihyeon Myeong1,14, Minho Lee2,14, Bawool Lee1,3,14, Joon Hyung Kim2, Yeji Nam4, Yeseul Choi5,6, Jeongmin Kim1, Se Young Jeon7,8, Haewon Shim2, Da-Ryung Jung9, Youngjin Shin2, Minsoo Jeong9, Byungmoo Oh1,3, Jaehun Jung2, Christine S. Kim1,3, Hyung Soo Han5,6,8,10, Jae-Ho Shin9, Yoon Hee Lee7,8,11, Nora Jee-Young Park8,12,13, Gun Oh Chong7,8,11 & Youngtae Jeong1,3
1Department of New Biology, DGIST, Daegu, Korea.
2Department of Life Science, Dongguk University, Gyeonggi-do, Korea.
3New Biology Research Center, DGIST, Daegu, Korea.
4School of Undergraduate Studies, DGIST, Daegu, Korea.
5Graduate Program, Department of Biomedical Science, Kyungpook National University School of Medicine, Daegu, Korea.
6BK21 Four Program, Kyungpook National University School of Medicine, Daegu, Korea.
7Department of Obstetrics and Gynecology, Kyungpook National University Chilgok Hospital, Daegu, Korea.
8Clinical Omics Institute, Kyungpook National University, Daegu, Korea.
9Department of Applied Biosciences, Kyungpook National University, Daegu, Korea.
10Department of Physiology, Kyungpook National University School of Medicine, Daegu, Korea.
11Department of Obstetrics and Gynecology, Kyungpook National University School of Medicine, Daegu, Korea.
12Department of Pathology, Kyungpook National University Chilgok Hospital, Daegu, Korea.
13Department of Pathology, Kyungpook National University School of Medicine, Daegu, Korea.
14These authors contributed equally: Jihyeon Myeong, Minho Lee, Bawool Lee.
Corresponding authors
Correspondence to Gun Oh Chong or Youngtae Jeong.
1Department of New Biology, DGIST, Daegu, Korea.
2Department of Life Science, Dongguk University, Gyeonggi-do, Korea.
3New Biology Research Center, DGIST, Daegu, Korea.
4School of Undergraduate Studies, DGIST, Daegu, Korea.
5Graduate Program, Department of Biomedical Science, Kyungpook National University School of Medicine, Daegu, Korea.
6BK21 Four Program, Kyungpook National University School of Medicine, Daegu, Korea.
7Department of Obstetrics and Gynecology, Kyungpook National University Chilgok Hospital, Daegu, Korea.
8Clinical Omics Institute, Kyungpook National University, Daegu, Korea.
9Department of Applied Biosciences, Kyungpook National University, Daegu, Korea.
10Department of Physiology, Kyungpook National University School of Medicine, Daegu, Korea.
11Department of Obstetrics and Gynecology, Kyungpook National University School of Medicine, Daegu, Korea.
12Department of Pathology, Kyungpook National University Chilgok Hospital, Daegu, Korea.
13Department of Pathology, Kyungpook National University School of Medicine, Daegu, Korea.
14These authors contributed equally: Jihyeon Myeong, Minho Lee, Bawool Lee.
Corresponding authors
Correspondence to Gun Oh Chong or Youngtae Jeong.
Abstract
Cervical cancer is the fourth most common female cancer, with the uterine ectocervix being the most commonly affected site. However, cervical stem cells, their differentiation, and their regulation remain poorly understood. Here, we report the isolation of a population enriched for human cervical stem cells and their regulatory mechanisms. Using single-cell RNA sequencing, we characterize the cellular heterogeneity of the human ectocervix and identify cluster-specific cell surface markers. By establishing normal and precancerous cervical organoids and an intralingual transplantation system, we show that ITGB4 and CD24 enable enrichment of human and murine ectocervical stem cells. We discover that Lactobacilli-derived lactic acid regulates cervical stem cells’ self-renewal and early tumorigenesis through the PI3K-AKT pathway and YAP1. Finally, we show that D-lactic acid suppresses growth of normal and precancerous organoids, while L-lactic acid does not. Our findings reveal roles of human cervical stem cells and microbial metabolites in cervical health and diseases.
논문정보
- Research article
- 2025년 03월 (BRIC 등록일 2025-03-10)
- 국내 연구진
- 의약학 > 재생의학
댓글 작성 로그인
팝업 닫기관련 링크
관련분야 논문보기
