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Adv. Sci., Mar 06 2025, | https://doi.org/10.1002/advs.202411191
Structural Diversity of Mitochondria in the Neuromuscular System across Development Revealed by 3D Electron Microscopy
J. Alexander Bae 1 *, Myung-Kyu Choi 2, Soungyub Ahn 2, Gwanho Ko 2, Daniel T Choe 2, Hyunsoo Yim 2, Ken C Nguyen 3, Jinseop S Kim 4, David H Hall 3, Junho Lee 1 2 *
1Research Institute of Basic Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
2Department of Biological Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
3Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, 10461, USA.
4Department of Biological Sciences, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
2Department of Biological Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
3Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, 10461, USA.
4Department of Biological Sciences, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
†J.A.B. and M.C. contributed equally to this work.
*Corresponding authors: correspondence to J. Alexander Bae or Junho Lee
Abstract
As an animal matures, its neural circuit undergoes alterations, yet the developmental changes in intracellular organelles to facilitate these changes is less understood. Using 3D electron microscopy and deep learning, the study develops semi-automated methods for reconstructing mitochondria in C. elegans and collected mitochondria reconstructions from normal reproductive stages and dauer, enabling comparative study on mitochondria structure within the neuromuscular system. It is found that various mitochondria structural properties in neurons correlate with synaptic connections and these properties are preserved across development in different neural circuits. To test the necessity of these universal mitochondria properties, the study examines the behavior in drp-1 mutants with impaired mitochondria fission and discovers that it causes behavioral deficits. Moreover, it is observed that dauer neurons display distinctive mitochondrial features, and mitochondria in dauer muscles exhibit unique reticulum-like structure. It is proposed that these specialized mitochondria structures may serve as an adaptive mechanism to support stage-specific behavioral and physiological needs.
논문정보
- Research article
- 2025년 03월 (BRIC 등록일 2025-03-10)
- 국내(교신)+국외 연구진
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