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Cell Syst., Feb 26 2025, S2405-4712(25)00036-5 | https://doi.org/10.1016/j.cels.2025.101203
Large-scale control over collective cell migration using light-activated epidermal growth factor receptors
Kevin Suh,1,2,5 Richard H. Thornton,2,3,5 Long Nguyen,2 Payam E. Farahani,1 Daniel J. Cohen,2,4,*and Jared E. Toettcher 2,3,6, *
1Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
2Omenn-Darling Bioengineering Institute, Princeton University, Princeton, NJ 08544, USA
3Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
4Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA
5These authors contributed equally
6Lead contact
2Omenn-Darling Bioengineering Institute, Princeton University, Princeton, NJ 08544, USA
3Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
4Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA
5These authors contributed equally
6Lead contact
*Corresponding authors: correspondence to Daniel J. Cohen or Jared E. Toettcher
Abstract
Receptor tyrosine kinases (RTKs) play key roles in coordinating cell movement at both single-cell and tissue scales. The recent development of optogenetic tools for controlling RTKs and their downstream signaling pathways suggests that these responses may be amenable to engineering-based control for sculpting tissue shape and function. Here, we report that a light-controlled epidermal growth factor (EGF) receptor (OptoEGFR) can be deployed in epithelial cells for precise, programmable control of long-range tissue movements. We show that in OptoEGFR-expressing tissues, light can drive millimeter-scale cell rearrangements to densify interior regions or produce rapid outgrowth at tissue edges. Light-controlled tissue movements are driven primarily by phosphoinositide 3-kinase (PI3K) signaling, rather than diffusible ligands, tissue contractility, or ERK kinase signaling as seen in other RTK-driven migration contexts. Our study suggests that synthetic, light-controlled RTKs could serve as a powerful platform for controlling cell positions and densities for diverse applications, including wound healing and tissue morphogenesis.
논문정보
- Research article
- 2025년 02월 (BRIC 등록일 2025-03-07)
- 국외 연구진
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