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Bioact. Mater., Available online 22 February 202 | https://doi.org/10.1016/j.bioactmat.2025.02.013
Precision repair of zone-specific meniscal injuries using a tunable extracellular matrix-based hydrogel system
Se-Hwan Lee a,d, Zizhao Li a,b, Ellen Y. Zhang a,b, Dong Hwa Kim a, Ziqi Huang c, Yuna Heo a, Sang Jin Lee c, Hyun-Wook Kang d, Jason A. Burdick b,e, Robert L. Mauck a,b,f, Su Chin Heo a,b,f
aMcKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, United States
bDepartment of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, 19104, United States
cBiofunctional Materials, Division of Applied Oral Sciences and Community Dental Care, Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, PR China
dDepartment of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919, Republic of Korea
eBioFrontiers Institute and Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, 80309, United States
fTranslational Musculoskeletal Research Center, Corporal Michael J Crescenz VA Medical Center, Philadelphia, PA, 19104, United States
Corresponding author : Su Chin Heo
aMcKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, United States
bDepartment of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, 19104, United States
cBiofunctional Materials, Division of Applied Oral Sciences and Community Dental Care, Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, PR China
dDepartment of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919, Republic of Korea
eBioFrontiers Institute and Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, 80309, United States
fTranslational Musculoskeletal Research Center, Corporal Michael J Crescenz VA Medical Center, Philadelphia, PA, 19104, United States
Corresponding author : Su Chin Heo
Abstract
Meniscus injuries present significant therapeutic challenges due to their limited self-healing capacity and the diverse biological and mechanical properties across the tissue. Conventional repair strategies do not replicate the complex zonal characteristics within the meniscus, resulting in suboptimal outcomes. In this study, we introduce an innovative fetal/adult and stiffness-tunable meniscus decellularized extracellular matrix (DEM)-based hydrogel system designed for precision repair of heterogeneous, zonal-dependent meniscus injuries. By synthesizing fetal and adult DEM hydrogels, we identified distinct cellular responses, including that hydrogels with adult meniscus-derived DEM promote more fibrochondrogenic phenotypes. The incorporation of methacrylated hyaluronic acid (MeHA) further refined the mechanical properties and injectability of the DEM-based hydrogels. The combination of fetal and adult DEM with MeHA allowed for precise tuning of stiffness, influencing cell differentiation and closely mimicking native tissue environments. In vivo tests confirmed the biocompatibility of hydrogels and their integration with native meniscus tissues. Furthermore, advanced 3D bioprinting techniques enabled the fabrication of hybrid hydrogels with biomaterial and mechanical gradients, effectively emulating the zonal properties of meniscus tissue and enhancing cell integration. This study represents a significant advance in meniscus tissue engineering, providing a promising platform for customized regenerative therapies across a range of heterogeneous fibrous connective tissues.
논문정보
- Research article
- 2025년 02월 (BRIC 등록일 2025-03-05)
- 국내+국외 연구진
- 바이오·의료융합 > 바이오소재
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