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University of Illinois-Urbana-Champaign

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Chem. Eng. J., Available online 25 February 2025, 160947 | https://doi.org/10.1016/j.cej.2025.160947 Biofilm comes back: Controlling regrowth by mitigating the cell-matrix interaction

Yu-Heng Deng ^a,1, Joo Hun Lee ^a,1, Myung-Joo Kim ^b, Hyunjoon Kong ^a,c,d,e,f

^aDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana- Champaign Urbana IL United States
^bDepartment of Prosthodontics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 110-749, South Korea
^cDepartment of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
^dBeckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
^eCarle Illinois College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
^fScott H. Fisher Multicellular Engineered Living Systems Theme, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States

¹These authors contributed equally to this work.
Corresponding author : Hyunjoon Kong

Abstract

Biofilms wield a notorious impact on biological infections and environmental sanitation in daily life. Tremendous efforts have been made to develop antimicrobial agents that effectively kill bacterial cells, but preventing biofilm reformation remains unsolved. For instance, a hydrogen peroxide (H2O2) and peracetic acid (PAA) mixture can inactivate almost 100 % of Pseudomonas aeruginosa cells in a biofilm within 10 min, but why do cells regrow and recover the biofilm within a day? We hypothesize that interaction between bacterial cells and extracellular polymeric substances (EPS) remaining during biofilm treatment is responsible for biofilm regrowth. This hypothesis is examined by quantifying the number of cells associated with a unit volume of EPS (Manders coefficient) and the mass of EPS associated with a cell in the P. aeruginosa biofilm by processing immunostained biofilm images with the BiofilmQ software. Interestingly, the H2O2 + PAA mixture increases the EPS mass associated with a single cell compared with untreated conditions while minimally affecting the Manders coefficient. To resolve this challenge, we devise a sequential strategy in which self-locomotive MnO2-doped diatoms are initially added to reduce the EPS volume, making bacterial cells more susceptible to the subsequently added H2O2 + PAA mixture. This strategy significantly reduces the mass of EPS associated with cells, thereby inhibiting biofilm regrowth over 2 months. Overall, the results of these studies provide valuable insights for developing advanced antibacterial strategies that can significantly improve an ability to control infection and biofouling across a diverse spectrum of household, healthcare, and industrial applications.

논문정보

형식Research article
게재일2025년 02월 (BRIC 등록일 2025-02-28)
연구진국내+국외 연구진
분야 바이오·의료융합 > 바이오소재

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