안경익 (경상국립대학교) | 한빛사논문 > 한빛사

한빛사논문

조회95

경상국립대학교

CV File 258 kb

Plant Commun., Feb 13 2025, S2590-3462(25)00046-X | https://doi.org/10.1016/j.xplc.2025.101284 The core morning clock component CCA1 positively regulates the expression of UPR target genes for ER stress recovery

Gyeongik Ahn^{1 5}, In Jung Jung^{2 5}, Gyeong-Im Shin ^{1 5}, Song Yi Jeong ¹, Myung Geun Ji ¹, Jin-Sung Huh ¹, Ji-Won Hwang ¹, Jeongsik Kim ³, Joon-Yung Cha ¹, Sang Yeol Lee ¹, Min Gab Kim^{4 *}, Woe-Yeon Kim^{1 *}

¹Division of Applied Life Science (BK21 four), PBRRC, PMBBRC, RILS & IALS, Gyeongsang National University, Jinju 52828, Republic of Korea
²National Institute of Wildlife Disease Control and Prevention (NIWDC), Ministry of Environment, Gwangju 62407, Republic of Korea
³Faculty of Science Education and Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju 63243, Republic of Korea
⁴College of Pharmacy and Research Institute of Pharmaceutical Science, PMBBRC, Gyeongsang National University, Jinju 52828, Republic of Korea

⁵These authors contributed equally.

^*Corresponding authors: correspondence to Min Gab Kim or Woe-Yeon Kim

Abstract

The endoplasmic reticulum (ER) is a cellular organelle responsible for protein synthesis and folding. When the protein folding capacity is exceeded, unfolded or misfolded proteins accumulate, causing ER stress and triggering the unfolded protein response (UPR) to restore ER proteostasis. Although UPR genes in plants are expressed in a diel cycle, the mechanisms by which the circadian clock regulates these genes are not well understood. Here, we demonstrate that ER stress sensitivity in root growth exhibits time-of-day phases and that the circadian clock regulates the expression of UPR target genes during ER stress. Notably, mutations in the core morning clock component CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) impair ER stress recovery. CCA1 forms a complex with the UPR modulator bZIP28 and acts as upstream regulator in ER stress recovery. Upon ER stress, CCA1 is stabilized and associates with bZIP28 at the ER stress response element of the BiP3 promoter, enhancing the ER stress response. Thus, CCA1 coordinates a time-dependent adaptive response to ER stress with bZIP28 to maintain ER proteostasis. Our results suggest that the circadian clock primes the timing and levels of ER chaperone expression to enhance ER stress tolerance.

논문정보

형식Research article
게재일2025년 02월 (BRIC 등록일 2025-03-12)
연구진국내 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기