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ACS Nano, Feb 15 2025, | https://doi.org/10.1021/acsnano.4c14735
Multilayer Adjuvanted Influenza Protein Nanoparticles Improve Intranasal Delivery and Antigen-Specific Immunity
Jaeyoung Park 1, Thomas Pho 1,2, Noopur Bhatnagar 3, Linh D Mai 1, Mariela R Rodriguez-Otero 1,2, Surya Sekhar Pal 3, Chau Thuy Tien Le 3, Sarah E Jenison 1, Chenyu Li 1, Grace A May 1, Marisa Arioka 4, Sang-Moo Kang 3, Julie A Champion 1,2
1School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.
2Bioengineering Program, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.
3Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, Georgia 30302, United States.
4Department of Chemistry, Tokyo University of Science, Shinjuku-ku, Tokyo 162-8601, Japan.
J.P. and T.P. contributed equally.
Corresponding Authors : Sang-Moo Kang, Julie A. Champion
1School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.
2Bioengineering Program, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.
3Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, Georgia 30302, United States.
4Department of Chemistry, Tokyo University of Science, Shinjuku-ku, Tokyo 162-8601, Japan.
J.P. and T.P. contributed equally.
Corresponding Authors : Sang-Moo Kang, Julie A. Champion
Abstract
Intranasal vaccination is a desired route for protection against influenza viruses by mucosal and systemic immunity. However, the nasal mucosa impedes the intranasal delivery of vaccines. Here, we formulated layer-by-layer (LBL) influenza vaccine nanoparticles for effective intranasal delivery by coating them with alternating mucoadhesive cationic chitosan and muco-inert anionic CpG adjuvants. The nanoparticle cores were formed by desolvating influenza M2e antigen and coating it with hemagglutinin (HA) antigen via biotin-streptavidin conjugation. LBL modification promoted nasal delivery and interaction with the resident immune cells. Intranasal administration with LBL nanoparticles significantly improved cellular and humoral immune responses against HA and M2e including high IgA titers, a hallmark of potent mucosal immunity and persistence of immune responses. Distinct trends for antigen-specific immune responses were observed for different routes of vaccination. The enhanced immune responses conferred mice protection against the influenza challenge and prominently reduced viral titers, demonstrating the effectiveness of intranasal LBL vaccine nanoparticles.
논문정보
- Research article
- 2025년 02월 (BRIC 등록일 2025-02-19)
- 국외 연구진
- 생명과학 > 생물공학
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