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Biosens. Bioelectron., Available online 13 February 2025, 117257 | https://doi.org/10.1016/j.bios.2025.117257
Cellular level cryo-neuromodulation using rapid and localized cooling device combined with microelectrode array
Jaehyun Kim 1, Jong Seung Lee 2, Soyeon Noh 4, Eunseok Seo 1, Jungchul Lee 5, Taesung Kim 4, Seung-Woo Cho 2,3, Gunho Kim 4, Sung Soo Kim 6, Jungyul Park 1
1Department of Mechanical Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea
2Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea
3Center for Nanomedicine, Institute for Basic Science (IBS), 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea
4Department of Mechanical Engineering, Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan 44919, Republic of Korea
5Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291, Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
6Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA
Corresponding authors: Seung-Woo Cho, Gunho Kim, Sung Soo Kim, Jungyul Park
1Department of Mechanical Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea
2Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea
3Center for Nanomedicine, Institute for Basic Science (IBS), 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea
4Department of Mechanical Engineering, Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan 44919, Republic of Korea
5Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291, Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
6Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA
Corresponding authors: Seung-Woo Cho, Gunho Kim, Sung Soo Kim, Jungyul Park
Abstract
Cryotherapy, a rapid and effective medical treatment utilizing low temperatures, has not been widely adopted in clinical practice due to a limited understanding of its mechanisms and efficacy. This challenge stems from the absence of methods for fast, precise, and localized spatiotemporal temperature control, as well as the lack of reliable real-time quantitative techniques for measuring and analyzing the effects of cooling. To address these limitations, this study introduces a cryo-neuromodulation platform that integrates a high-speed precision cooling device with a microelectrode array (MEA) system. This platform enables the investigation of cellular-level cryo-modulation of neuronal activity and its effects on surrounding cells, providing a novel framework for advancing research in cryotherapy and neuromodulation. Experiments show that neurons recovered fully within 1 minute of cooling with a fast-cooling rate (-20°C/s at cooling) and that silenced neurons can influence distant cells via a well-organized network. Extended cooling durations (e.g., 10 minutes) resulted in altered neuronal dynamics, including delayed recovery and reduced burst activity, highlighting the importance of precise control over cooling parameters. This device offers reversible neural control, with potential applications in both research and clinical settings, such as anesthesia, pain management and treatment of neurological disorders like neocortical seizures.
논문정보
- Research article
- 2025년 02월 (BRIC 등록일 2025-02-14)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 다학제 융합/복합
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