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Nat. Commun., 12 February 2025 | https://doi.org/10.1038/s41467-025-56856-0
Oligonucleotide subsets selection by single nucleotide resolution barcode identification
Woojin Kim1,6, Mingweon Chon2,6, Yoonhae Koh1,6, Hansol Choi2,3,5,6, Eunjin Choi1, Hyewon Park4, Yushin Jung4, Taehoon Ryu4, Sunghoon Kwon2,3 & Yeongjae Choi1
1School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
2Department of Electrical and Computer Engineering, Seoul National University, Seoul, Republic of Korea.
3Bio-MAX Institute, Seoul National University, Seoul, Republic of Korea.
4ATG Lifetech Inc., Seoul, Republic of Korea.
5Present address: Department of Biological Chemistry and Molecular Pharmacology (BCMP), Harvard Medical School, Boston, MA, USA.
6These authors contributed equally: Woojin Kim, Mingweon Chon, Yoonhae Koh, Hansol Choi.
Corresponding authors
Correspondence to Sunghoon Kwon or Yeongjae Choi.
1School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
2Department of Electrical and Computer Engineering, Seoul National University, Seoul, Republic of Korea.
3Bio-MAX Institute, Seoul National University, Seoul, Republic of Korea.
4ATG Lifetech Inc., Seoul, Republic of Korea.
5Present address: Department of Biological Chemistry and Molecular Pharmacology (BCMP), Harvard Medical School, Boston, MA, USA.
6These authors contributed equally: Woojin Kim, Mingweon Chon, Yoonhae Koh, Hansol Choi.
Corresponding authors
Correspondence to Sunghoon Kwon or Yeongjae Choi.
Abstract
Effective subset selection from complex oligonucleotide libraries is crucial for genomics, synthetic biology, and DNA data storage. The polymerase chain reaction, foundational for amplifying target subsets is limited by primer design and length for specificity, which constrains the scalability of oligo libraries and increases the synthesis burden for primers. We introduce an oligo subset selection methodology that utilizes sequence-specific cyclic nucleotide synthesis and blocking of the template oligos. This approach eliminates the need for primers for selective hybridization and enables the encoding and selection of hundreds of subsets with barcode lengths of fewer than five nucleotides. Moreover, cyclic selection enables a hierarchical data structure in the oligo library, enhancing the programmability. This advancement offers a scalable and cost-effective solution for handling complex oligo libraries.
논문정보
- Research article
- 2025년 02월 (BRIC 등록일 2025-02-13)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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