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Adv. Healthc. Mater., Feb 10 2025, | https://doi.org/10.1002/adhm.202403118 Enhanced Bioprinting of 3D Corneal Stroma Patches with Reliability, Assessing Product Consistency and Quality through Optimized Electron Beam Sterilization

Jungbin Yoon¹, Yeon-Ju Lee ², Minji Kim¹, Ju Young Park ², Jinah Jang^1,2,3,4,5

¹Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, South Korea.
²BioBricks Co., Ltd, Pohang, 37673, South Korea.
³Department of Convergence IT Engineering, POSTECH, Pohang, 37673, South Korea.
⁴School of Interdisciplinary Bioscience and Bioengineering, POSTECH, Pohang, 37673, South Korea.
⁵Institute of Convergence Science, Yonsei University, Seoul, 220-710, South Korea.

Corresponding Author : Jinah Jang

Abstract

This study focuses on the optimization of sterilization methods for bioprinted three-dimensional (3D) corneal stroma patches prepared using cornea-derived decellularized extracellular matrix (Co-dECM) hydrogels and human keratocytes, with the aim of enhancing clinical applications in corneal tissue engineering. An essential aspect of this study is to refine the sterilization processes, particularly focusing on electron beam (EB) sterilization, to maintain the structural and functional integrity of the Co-dECM hydrogels while ensuring sterility. The study reveals that EB sterilization outperformed traditional methods like ethylene oxide (EtO) gas and autoclaving, which tend to degrade the biochemical properties of hydrogels. By optimizing the EB-sterilization process, the essential mechanical and biochemical characteristics needed for successful 3D bioprinting are retained, reducing batch variability in bioprinted 3D corneal stroma patches. Consistency in production is vital for meeting regulatory standards and ensuring patient safety. Moreover, the study investigates the immunomodulatory properties of sterilized hydrogels, emphasizing their potential to minimize inflammatory responses, which is crucial for maintaining keratocyte phenotype. These findings significantly advance biomedical engineering by providing a sterilization method that preserves material integrity, minimizes immunogenicity, and supports the clinical translation of bioprinted corneal stroma patches, offering a promising alternative to donor transplants and synthetic substitutes.

논문정보

형식Research article
게재일2025년 02월 (BRIC 등록일 2025-02-17)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

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