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Trac-Trends Anal. Chem., Available online 31 January 2025 | https://doi.org/10.1016/j.trac.2025.118164
Harnessing CRISPR technology for next-generation microphysiological systems
Sojin Song a,1, Hyun Wook Kang b,1, Minjin Kang c, Seok Chung a,b,d, Nakwon Choi a,c,d,e,f,g, Hong Nam Kim a,h, Young Joon Sung i
aCenter for Brain Technology, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, 02792, South Korea
bSchool of Mechanical Engineering, Korea University, Seoul, 02841, South Korea
cInstitute of Human Genetics, College of Medicine, Korea University, Seoul, 02841, South Korea
dKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, South Korea
eDepartment of Biomedical Sciences, College of Medicine, Korea University, Seoul, 02841, South Korea
fDepartment of Convergence Medicine, College of Medicine, Korea University, Seoul, 02841, South Korea
gResearch Institute of Convergence Biomedical Science, College of Medicine, Korea University, Seoul, 02841, South Korea
hDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul, 02792, South Korea
iDepartment of Chemical and Biological Engineering, Sookmyung Women’s University, Seoul, 04310, South Korea
1These authors contributed equally to this manuscript.
Corresponding authors: Sojin Song, Seok Chung, Nakwon Choi, Hong Nam Kim, Young Joon Sung
aCenter for Brain Technology, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, 02792, South Korea
bSchool of Mechanical Engineering, Korea University, Seoul, 02841, South Korea
cInstitute of Human Genetics, College of Medicine, Korea University, Seoul, 02841, South Korea
dKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, South Korea
eDepartment of Biomedical Sciences, College of Medicine, Korea University, Seoul, 02841, South Korea
fDepartment of Convergence Medicine, College of Medicine, Korea University, Seoul, 02841, South Korea
gResearch Institute of Convergence Biomedical Science, College of Medicine, Korea University, Seoul, 02841, South Korea
hDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul, 02792, South Korea
iDepartment of Chemical and Biological Engineering, Sookmyung Women’s University, Seoul, 04310, South Korea
1These authors contributed equally to this manuscript.
Corresponding authors: Sojin Song, Seok Chung, Nakwon Choi, Hong Nam Kim, Young Joon Sung
Abstract
Microphysiological systems (MPSs) bridge traditional cell cultures and animal models, recapitulating human organ functions and disease characteristics. Despite their promise, MPSs face limitations like genetic diversity and disease phenotype replication. Recent advancements have integrated CRISPR/Cas gene editing into MPSs to address these challenges and enhance disease-specific models. This review explores the latest trends in MPS and CRISPR technologies, examining their fundamentals and current state. It details the utilization of CRISPR/Cas within in vitro systems, including traditional cell cultures and MPSs, focusing on mimicking organ structures and functions like the blood-brain barrier, brain, and kidney and optimizing cell culture conditions. Our review highlights applications of CRISPR-applied MPSs in translational science, such as studying disease mechanisms, identifying therapeutic targets, and developing drugs. We also discuss CRISPR-MPS platforms' challenges, limitations, and future perspectives, emphasizing their potential to meet unmet needs in emerging MPS developments.
논문정보
- Research article
- 2025년 01월 (BRIC 등록일 2025-02-11)
- 국내 연구진
- 생명과학 > 생물공학
관련 보도자료
CRISPR 기술 기반 미세생리시스템 융합연구 분석
유전자 편집 기술을 활용해 인체 조직과 유사하게 구현된 미세생리시스템(MPS)이 신약 개발과 질병 연구에서 주목받고 있다. 고려대학교 의과대학 융합의학교실 최낙원 교수 연구팀(고려대 기계공학부 정석 교수, 한국과학기술연구원 뇌과학연구소 김홍남, 송소진 박사, 숙명여대 화공생명공학부 성영준 교수)이 유전자 가위로 알려진 CRISPR/Cas(크리스퍼/카스) 기술과 미세생리시스템을 융합한 최신 연구동향을 분석하고, 이를 신약 개발과 맞춤형 치료에 적용할 수 있는 가능성을 제시했다.
- Bio통신원
- 2025. 03. 14
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