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Nat. Commun., 06 February 2025 | https://doi.org/10.1038/s41467-025-56373-0
Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arrays
Yeon-Woo Cho1,2,4, Min-Ji Kang1,4, Joon-Ha Park1, Yun-Sik Eom1,2 & Tae-Hyung Kim1,3
1Department of Intelligent Precision Healthcare Convergence, Institute for Cross-disciplinary Studies (ICS) , Sungkyunkwan University (SKKU), Suwon, Gyeonggi 16419, Republic of Korea.
2School of Integrative Engineering, Chung-Ang University, 84 Heukseuk-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
3Department of Biomedical Engineering, ICS, SKKU, Suwon, Gyeonggi 16419, Republic of Korea.
4These authors contributed equally: Yeon-Woo Cho, Min-Ji Kang
Corresponding author
Correspondence to Tae-Hyung Kim.
1Department of Intelligent Precision Healthcare Convergence, Institute for Cross-disciplinary Studies (ICS) , Sungkyunkwan University (SKKU), Suwon, Gyeonggi 16419, Republic of Korea.
2School of Integrative Engineering, Chung-Ang University, 84 Heukseuk-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
3Department of Biomedical Engineering, ICS, SKKU, Suwon, Gyeonggi 16419, Republic of Korea.
4These authors contributed equally: Yeon-Woo Cho, Min-Ji Kang
Corresponding author
Correspondence to Tae-Hyung Kim.
Abstract
Improved in vitro models are needed for regenerative therapy and drug screening. Here, we report on functionally aligned nanoparticle-trapped nanopattern arrays for spatially controlled, precise mesenchymal stem cell differentiation on a single substrate. The arrays comprise nanohole and nanoline arrays fabricated through interference lithography and selectively capture of UiO-67 metal–organic frameworks on nanoline arrays with a 99.8% efficiency using an optimised asymmetric spin-coating method. The UiO-67 metal–organic frameworks contain three osteogenic differentiation factors for sustained release over four weeks. The combination of differentiation factors and patterned array allows for generation of adipocytes, osteoblasts, and adipocyte–osteoblast mixtures on nanohole arrays, nanoline arrays, and at the nanohole–nanoline interface, respectively, with mature osteoblasts exhibiting higher marker expression and mineralisation. The sustained release patterned array holds potential for constructing advanced therapeutic and disease state in vitro cellular models.
논문정보
- Research article
- 2025년 02월 (BRIC 등록일 2025-02-06)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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