한빛사
>
한빛사논문
한빛사논문
- 조회221
Redox Biol., Available online 27 January 2025 | https://doi.org/10.1016/j.redox.2025.103512
Norharmane prevents muscle aging via activation of SKN-1/NRF2 stress response pathways
Farida S. Nirmala a,c, Hyunjung Lee a, Yejin Cho a, Min Young Um b,c, Hyo Deok Seo c, Chang Hwa Jung a,c, Jeong-Hoon Hahm a, Jiyun Ahn a,c
aAging and Metabolism Research Group, Korea Food Research Institute, Wanju-gun, South Korea
bFunctional Food Materials Research Group, Korea Food Research Institute, Wanju-gun, South Korea
cDepartment of Food Biotechnology, Korea University of Science and Technology, Daejeon-si, South Korea
Corresponding authors: Jeong-Hoon Hahm, Jiyun Ahn
aAging and Metabolism Research Group, Korea Food Research Institute, Wanju-gun, South Korea
bFunctional Food Materials Research Group, Korea Food Research Institute, Wanju-gun, South Korea
cDepartment of Food Biotechnology, Korea University of Science and Technology, Daejeon-si, South Korea
Corresponding authors: Jeong-Hoon Hahm, Jiyun Ahn
Abstract
Sarcopenia, the age-related decline in muscle mass and function, is a significant contributor to increased frailty and mortality in the elderly. Currently, no FDA-approved treatment exists for sarcopenia. Here, we identified norharmane (NR), a β-carboline alkaloid, as a potential therapeutic agent for mitigating muscle aging. We aimed to determine the ability of NR to delay muscle aging in Caenorhabditis elegans (C. elegans), mouse, and muscle cells in mice and humans. NR treatment improved swimming ability and increased the maximum velocity in aged C. elegans. Transcriptomic analysis revealed that NR upregulated detoxification genes in C. elegans, including cytochrome P450, UGT, and GST enzymes. NR-induced benefits were dependent on the SKN-1/Nrf2 stress response pathway. In mammalian models, NR delayed cellular senescence in human skeletal muscle myoblasts and enhanced myogenesis in C2C12 cells and primary aged myoblasts. NR supplementation in aged mice prevented muscle loss, improved muscle function, and reduced markers of cellular senescence. We found that the p38 MAPK pathway mediated NR activation of Nrf2 by disrupting the Nrf2-Keap1 interaction. NR also improved oxygen consumption rates and promoted mitochondrial biogenesis. These findings suggest that NR is a promising candidate for preventing sarcopenia and improving muscle health.
논문정보
- Research article
- 2025년 01월 (BRIC 등록일 2025-02-03)
- 국내 연구진
- 생명과학 > 노화/암생물학
댓글 작성 로그인
팝업 닫기관련 링크
소속기관 논문보기
관련분야 논문보기
